Proliferative Typhlocolitis With Multinucleated Giant Cells: A Nonspecific Enteropathy in Immunodeficient Sentinel Mice

Kerriann M. Casey, Amanda L. Johnson, Melea N. Hunrath, Jenelle K. Fraser, Nicole C. McCowan, Katherine Wasson, Rosalinda A. Doty, Stephen M Griffey, Denise Imai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Beginning in 2015, athymic nude sentinel mice from conventional, medium-, and high-security facilities presented to the Comparative Pathology Laboratory (CPL) with weight loss, diarrhea, and/or rectal prolapse. Regardless of whether clinical signs were present or absent, the gross observation of ceco-colonic thickening corresponded histologically to pleocellular typhlocolitis with mucosal hyperplasia and lamina proprial multinucleated cells. A subset of affected sentinels exhibited granulomatous serositis and hepatosplenic necrosis with multinucleated cells. Initial suspicion of mouse hepatitis virus infection was excluded by polymerase chain reaction, electron microscopy, and serology. Multinucleated giant cells were confirmed as macrophages by positive immunoreactivity to Mac-3 and Iba-1 and negative immunoreactivity to pancytokeratin. From conventional and medium-security facilities, Helicobacter species were identified in 40 of 143 (27.9%) mice, with H. hepaticus accounting for 72.5% of identified Helicobacter species. Other agents included opportunistic bacterial infection (41/145, 28.3%), murine norovirus (16/106, 15.1%), and pinworms (2/146, 1.4%). From high-security facilities, only Enterobacter cloacae was identified (2/13, 15.4%), and no evidence of Helicobacter sp., murine norovirus, or pinworms was present. No potentially infectious disease agent(s) was identified in 71 of 146 (48.6%) affected nude sentinels from conventional and medium-security facilities and 11 of 13 (84.6%) affected nude sentinels from high-security facilities. No statistically significant differences in histologic lesion scores were identified between Helicobacter-positive and Helicobacter-negative mice. Thus, proliferative typhlocolitis with multinucleated giant cells was considered a nonspecific histologic pattern associated with a variety of primary and opportunistic pathogens in athymic nude mice.

Original languageEnglish (US)
Pages (from-to)157-168
Number of pages12
JournalVeterinary Pathology
Volume56
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Helicobacter
giant cells
Giant Cells
digestive system diseases
Nude Mice
Enterobius
mice
Norovirus
Serositis
Murine hepatitis virus
Helicobacter hepaticus
Rectal Prolapse
Enterobacter cloacae
Opportunistic Infections
Virus Diseases
Serology
Bacterial Infections
Hyperplasia
Communicable Diseases
Weight Loss

Keywords

  • athymic nude
  • Helicobacter
  • mouse hepatitis virus
  • multinucleated giant cells
  • proliferative typhlocolitis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Proliferative Typhlocolitis With Multinucleated Giant Cells : A Nonspecific Enteropathy in Immunodeficient Sentinel Mice. / Casey, Kerriann M.; Johnson, Amanda L.; Hunrath, Melea N.; Fraser, Jenelle K.; McCowan, Nicole C.; Wasson, Katherine; Doty, Rosalinda A.; Griffey, Stephen M; Imai, Denise.

In: Veterinary Pathology, Vol. 56, No. 1, 01.01.2019, p. 157-168.

Research output: Contribution to journalArticle

Casey, Kerriann M. ; Johnson, Amanda L. ; Hunrath, Melea N. ; Fraser, Jenelle K. ; McCowan, Nicole C. ; Wasson, Katherine ; Doty, Rosalinda A. ; Griffey, Stephen M ; Imai, Denise. / Proliferative Typhlocolitis With Multinucleated Giant Cells : A Nonspecific Enteropathy in Immunodeficient Sentinel Mice. In: Veterinary Pathology. 2019 ; Vol. 56, No. 1. pp. 157-168.
@article{ede266a449d14953903f169bc271ed89,
title = "Proliferative Typhlocolitis With Multinucleated Giant Cells: A Nonspecific Enteropathy in Immunodeficient Sentinel Mice",
abstract = "Beginning in 2015, athymic nude sentinel mice from conventional, medium-, and high-security facilities presented to the Comparative Pathology Laboratory (CPL) with weight loss, diarrhea, and/or rectal prolapse. Regardless of whether clinical signs were present or absent, the gross observation of ceco-colonic thickening corresponded histologically to pleocellular typhlocolitis with mucosal hyperplasia and lamina proprial multinucleated cells. A subset of affected sentinels exhibited granulomatous serositis and hepatosplenic necrosis with multinucleated cells. Initial suspicion of mouse hepatitis virus infection was excluded by polymerase chain reaction, electron microscopy, and serology. Multinucleated giant cells were confirmed as macrophages by positive immunoreactivity to Mac-3 and Iba-1 and negative immunoreactivity to pancytokeratin. From conventional and medium-security facilities, Helicobacter species were identified in 40 of 143 (27.9{\%}) mice, with H. hepaticus accounting for 72.5{\%} of identified Helicobacter species. Other agents included opportunistic bacterial infection (41/145, 28.3{\%}), murine norovirus (16/106, 15.1{\%}), and pinworms (2/146, 1.4{\%}). From high-security facilities, only Enterobacter cloacae was identified (2/13, 15.4{\%}), and no evidence of Helicobacter sp., murine norovirus, or pinworms was present. No potentially infectious disease agent(s) was identified in 71 of 146 (48.6{\%}) affected nude sentinels from conventional and medium-security facilities and 11 of 13 (84.6{\%}) affected nude sentinels from high-security facilities. No statistically significant differences in histologic lesion scores were identified between Helicobacter-positive and Helicobacter-negative mice. Thus, proliferative typhlocolitis with multinucleated giant cells was considered a nonspecific histologic pattern associated with a variety of primary and opportunistic pathogens in athymic nude mice.",
keywords = "athymic nude, Helicobacter, mouse hepatitis virus, multinucleated giant cells, proliferative typhlocolitis",
author = "Casey, {Kerriann M.} and Johnson, {Amanda L.} and Hunrath, {Melea N.} and Fraser, {Jenelle K.} and McCowan, {Nicole C.} and Katherine Wasson and Doty, {Rosalinda A.} and Griffey, {Stephen M} and Denise Imai",
year = "2019",
month = "1",
day = "1",
doi = "10.1177/0300985818798106",
language = "English (US)",
volume = "56",
pages = "157--168",
journal = "Veterinary Pathology",
issn = "0300-9858",
publisher = "SAGE Publications Ltd",
number = "1",

}

TY - JOUR

T1 - Proliferative Typhlocolitis With Multinucleated Giant Cells

T2 - A Nonspecific Enteropathy in Immunodeficient Sentinel Mice

AU - Casey, Kerriann M.

AU - Johnson, Amanda L.

AU - Hunrath, Melea N.

AU - Fraser, Jenelle K.

AU - McCowan, Nicole C.

AU - Wasson, Katherine

AU - Doty, Rosalinda A.

AU - Griffey, Stephen M

AU - Imai, Denise

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Beginning in 2015, athymic nude sentinel mice from conventional, medium-, and high-security facilities presented to the Comparative Pathology Laboratory (CPL) with weight loss, diarrhea, and/or rectal prolapse. Regardless of whether clinical signs were present or absent, the gross observation of ceco-colonic thickening corresponded histologically to pleocellular typhlocolitis with mucosal hyperplasia and lamina proprial multinucleated cells. A subset of affected sentinels exhibited granulomatous serositis and hepatosplenic necrosis with multinucleated cells. Initial suspicion of mouse hepatitis virus infection was excluded by polymerase chain reaction, electron microscopy, and serology. Multinucleated giant cells were confirmed as macrophages by positive immunoreactivity to Mac-3 and Iba-1 and negative immunoreactivity to pancytokeratin. From conventional and medium-security facilities, Helicobacter species were identified in 40 of 143 (27.9%) mice, with H. hepaticus accounting for 72.5% of identified Helicobacter species. Other agents included opportunistic bacterial infection (41/145, 28.3%), murine norovirus (16/106, 15.1%), and pinworms (2/146, 1.4%). From high-security facilities, only Enterobacter cloacae was identified (2/13, 15.4%), and no evidence of Helicobacter sp., murine norovirus, or pinworms was present. No potentially infectious disease agent(s) was identified in 71 of 146 (48.6%) affected nude sentinels from conventional and medium-security facilities and 11 of 13 (84.6%) affected nude sentinels from high-security facilities. No statistically significant differences in histologic lesion scores were identified between Helicobacter-positive and Helicobacter-negative mice. Thus, proliferative typhlocolitis with multinucleated giant cells was considered a nonspecific histologic pattern associated with a variety of primary and opportunistic pathogens in athymic nude mice.

AB - Beginning in 2015, athymic nude sentinel mice from conventional, medium-, and high-security facilities presented to the Comparative Pathology Laboratory (CPL) with weight loss, diarrhea, and/or rectal prolapse. Regardless of whether clinical signs were present or absent, the gross observation of ceco-colonic thickening corresponded histologically to pleocellular typhlocolitis with mucosal hyperplasia and lamina proprial multinucleated cells. A subset of affected sentinels exhibited granulomatous serositis and hepatosplenic necrosis with multinucleated cells. Initial suspicion of mouse hepatitis virus infection was excluded by polymerase chain reaction, electron microscopy, and serology. Multinucleated giant cells were confirmed as macrophages by positive immunoreactivity to Mac-3 and Iba-1 and negative immunoreactivity to pancytokeratin. From conventional and medium-security facilities, Helicobacter species were identified in 40 of 143 (27.9%) mice, with H. hepaticus accounting for 72.5% of identified Helicobacter species. Other agents included opportunistic bacterial infection (41/145, 28.3%), murine norovirus (16/106, 15.1%), and pinworms (2/146, 1.4%). From high-security facilities, only Enterobacter cloacae was identified (2/13, 15.4%), and no evidence of Helicobacter sp., murine norovirus, or pinworms was present. No potentially infectious disease agent(s) was identified in 71 of 146 (48.6%) affected nude sentinels from conventional and medium-security facilities and 11 of 13 (84.6%) affected nude sentinels from high-security facilities. No statistically significant differences in histologic lesion scores were identified between Helicobacter-positive and Helicobacter-negative mice. Thus, proliferative typhlocolitis with multinucleated giant cells was considered a nonspecific histologic pattern associated with a variety of primary and opportunistic pathogens in athymic nude mice.

KW - athymic nude

KW - Helicobacter

KW - mouse hepatitis virus

KW - multinucleated giant cells

KW - proliferative typhlocolitis

UR - http://www.scopus.com/inward/record.url?scp=85059742509&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059742509&partnerID=8YFLogxK

U2 - 10.1177/0300985818798106

DO - 10.1177/0300985818798106

M3 - Article

C2 - 30222063

AN - SCOPUS:85059742509

VL - 56

SP - 157

EP - 168

JO - Veterinary Pathology

JF - Veterinary Pathology

SN - 0300-9858

IS - 1

ER -