Proliferation response to interleukin-2 and Jak/Stat activation of T cells immortalized by human T-cell lymphotropic virus type 1 is independent of open reading frame I expression

Nathaniel D. Collins, Celine D'Souza, Björn Albrecht, Michael D. Robek, Lee Ratner, Wei Ding, Patrick L. Green, Michael Dale Lairmore

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Abstract

Human T-cell lymphotropic virus type 1 (HTLV-1), a complex retrovirus, encodes a hydrophobic 12-kD protein from pX open reading frame (ORF) I that localizes to cellular endomembranes and contains four minimal SH3 binding motifs (PXXP). We have demonstrated the importance of ORF I expression in the establishment of infection and hypothesize that p121 has a role in T-cell activation. In this study, we tested interleukin-2 (IL-2) receptor expression, IL-2-mediated proliferation, and Jak/Stat activation in T-cell lines immortalized with either wild-type or ORF I mutant clones of HTLV-1. All cell lines exhibited typical patterns of T-cell markers and maintained mutation fidelity. No significant differences between cell lines were observed in IL-2 receptor chain (α,β, or γ) expression, in IL-2-mediated proliferation, or in IL-2-induced phosphorylated forms of Stat3, Stat5, Jak1, or Jak3. The expression of ORF I is more likely to play a role in early HTLV- 1 infection, such as in the activation of quiescent T cells in vivo.

Original languageEnglish (US)
Pages (from-to)9642-9649
Number of pages8
JournalJournal of Virology
Volume73
Issue number11
StatePublished - 1999
Externally publishedYes

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Primate T-lymphotropic virus 1
Human T-lymphotropic virus 1
interleukin-2
Open Reading Frames
Interleukin-2
open reading frames
T-lymphocytes
T-Lymphocytes
cell lines
Interleukin-2 Receptors
Retroviridae
Cell Line
Deltaretrovirus Infections
receptors
infection
clones
mutation
Clone Cells
mutants
Mutation

ASJC Scopus subject areas

  • Immunology

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Proliferation response to interleukin-2 and Jak/Stat activation of T cells immortalized by human T-cell lymphotropic virus type 1 is independent of open reading frame I expression. / Collins, Nathaniel D.; D'Souza, Celine; Albrecht, Björn; Robek, Michael D.; Ratner, Lee; Ding, Wei; Green, Patrick L.; Lairmore, Michael Dale.

In: Journal of Virology, Vol. 73, No. 11, 1999, p. 9642-9649.

Research output: Contribution to journalArticle

Collins, Nathaniel D. ; D'Souza, Celine ; Albrecht, Björn ; Robek, Michael D. ; Ratner, Lee ; Ding, Wei ; Green, Patrick L. ; Lairmore, Michael Dale. / Proliferation response to interleukin-2 and Jak/Stat activation of T cells immortalized by human T-cell lymphotropic virus type 1 is independent of open reading frame I expression. In: Journal of Virology. 1999 ; Vol. 73, No. 11. pp. 9642-9649.
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AU - Ratner, Lee

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