Proliferation indices in spontaneous canine lung cancer: Proliferating cell nuclear antigen (PCNA), Ki-67 (MIB1) and mitotic counts

Stephen M Griffey, S. A. Kraegel, B. R. Madewell

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27 Citations (Scopus)

Abstract

Proliferation indices were measured for specimens from 55 spontaneous canine lung tumours, collected by surgical biopsy from clinical patients and archived in paraffin wax blocks. These indices were then related to the mitotic index and histological type of the tumour. Proliferating cell nuclear antigen (PCNA) and Ki-67 (MIB1) proteins were detected immunohistochemically with a biotin-streptavidin amplified detection system on a representative tissue section from each tumour. Five adjacent, non-overlapping fields were selected at random, and 200 cells per field were examined in each section. For PCNA, cells were classified subjectively into negative, weak or strong reactively groups, based on nuclear staining. MIB1 cells were classified as negative or positive, based on nuclear staining. Mitotic figures were counted in anti-PCNA-labelled and anti-MIB1-labelled sections, in the same tumour areas as those in which indices were established. Mitotic counts were also done on haematoxylin and eosin-stained tissue sections. Linear regression analysis showed that all PCNA, MIB1 and mitotic indices had highly significant positive correlations (P < 0.0001) with each other. Adenosquamous and squamous cell carcinomas differed from other histological tumour types in having significantly higher proliferation indices. These data suggest that growth rates for lung tumours in the dog vary according to histological type. On the basis of differences in proliferation indices and the distribution of immunoreactivity between histological subtypes in this study, it would seem that immunohistochemical detection of PCNA and MIB/1 reactivity and analysis of mitotic figures in routinely processed tissues may be useful in the diagnosis of lung tumours.

Original languageEnglish (US)
Pages (from-to)321-332
Number of pages12
JournalJournal of Comparative Pathology
Volume120
Issue number4
DOIs
StatePublished - May 1999

Fingerprint

proliferating cell nuclear antigen
Proliferating Cell Nuclear Antigen
lung neoplasms
Canidae
Lung Neoplasms
dogs
neoplasms
Neoplasms
Mitotic Index
paraffin wax
Lung
streptavidin
squamous cell carcinoma
cells
biotin
Staining and Labeling
biopsy
Streptavidin
Waxes
regression analysis

ASJC Scopus subject areas

  • Animal Science and Zoology

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Proliferation indices in spontaneous canine lung cancer : Proliferating cell nuclear antigen (PCNA), Ki-67 (MIB1) and mitotic counts. / Griffey, Stephen M; Kraegel, S. A.; Madewell, B. R.

In: Journal of Comparative Pathology, Vol. 120, No. 4, 05.1999, p. 321-332.

Research output: Contribution to journalArticle

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