Progressive kidney degeneration in mice lacking tensin

Su Hao Lo, Qian Chun Yu, Linda Degenstein, Lan Bo Chen, Elaine Fuchs

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Tensin is a focal adhesion phosphoprotein that binds to F-actin and contains a functional Src homology 2 domain. To explore the biological functions of tensin, we cloned the mouse tensin gene, determined its program of expression, and used gene targeting to generate mice lacking tensin. Even though tensin is expressed in many different tissues during embryogenesis, tensin null mice developed normally and appeared healthy postnatally for at least several months. Over time, -/- mice became frail because of abnormalities in their kidneys, an organ that expresses high levels of tensin. Mice with overt signs of weakness exhibited signs of renal failure and possessed multiple large cysts in the proximal kidney tubules, but even in tensin null mice with normal blood analysis, cysts were prevalent. Ultrastructurally, noncystic areas showed typical cell-matrix junctions that readily labeled with antibodies against other focal adhesion molecules. In abnormal regions, cell-matrix junctions were disrupted and tubule cells lacked polarity. Taken together, our data imply that, in the kidney, loss of tensin leads to a weakening, rather than a severing, of focal adhesion. All other tissues appeared normal, suggesting that, in most cases, tensin's diverse functions are redundant and may be compensated for by other focal adhesion proteins.

Original languageEnglish (US)
Pages (from-to)1349-1361
Number of pages13
JournalJournal of Cell Biology
Volume136
Issue number6
DOIs
StatePublished - Mar 24 1997
Externally publishedYes

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Kidney
Focal Adhesions
Cell-Matrix Junctions
Cysts
Tensins
Cell Polarity
Proximal Kidney Tubule
src Homology Domains
Gene Targeting
Phosphoproteins
Embryonic Development
Renal Insufficiency
Actins
Antibodies
Genes
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Lo, S. H., Yu, Q. C., Degenstein, L., Chen, L. B., & Fuchs, E. (1997). Progressive kidney degeneration in mice lacking tensin. Journal of Cell Biology, 136(6), 1349-1361. https://doi.org/10.1083/jcb.136.6.1349

Progressive kidney degeneration in mice lacking tensin. / Lo, Su Hao; Yu, Qian Chun; Degenstein, Linda; Chen, Lan Bo; Fuchs, Elaine.

In: Journal of Cell Biology, Vol. 136, No. 6, 24.03.1997, p. 1349-1361.

Research output: Contribution to journalArticle

Lo, SH, Yu, QC, Degenstein, L, Chen, LB & Fuchs, E 1997, 'Progressive kidney degeneration in mice lacking tensin', Journal of Cell Biology, vol. 136, no. 6, pp. 1349-1361. https://doi.org/10.1083/jcb.136.6.1349
Lo, Su Hao ; Yu, Qian Chun ; Degenstein, Linda ; Chen, Lan Bo ; Fuchs, Elaine. / Progressive kidney degeneration in mice lacking tensin. In: Journal of Cell Biology. 1997 ; Vol. 136, No. 6. pp. 1349-1361.
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