Progress in OCT-based functional cellular resolution retinal imaging in mice: Application of temporal Super Averaging

Robert J. Zawadzki, Pengfei Zhang, Eric B. Miller, Suman K. Manna, Ratheesh K. Meleppat, Marie E. Burns, Edward N. Pugh

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Over the last two decades optical coherence tomography (OCT) has become a clinical gold standard in diagnostic and management of many retinal diseases. Following that success, OCT is now becoming a standard tool in studies of animal models of human retinal diseases allowing longitudinal evaluation of disease progression and responses to therapeutic intervention in the single animal. Recent progress in OCT data acquisition and processing allowed successful cellular resolution detection of outer and inner retina functional response to light stimulation both in humans and experimental animals. In this manuscript, we will describe our latest effort of improving cellular contrast in mouse functional retinal imaging by application of volumetric registration and speckle contrast reduction by temporal averaging of volumetric data sets: i.e., temporal Super Averaging. As an example, the current configuration of our multimodal Scanning Light Ophthalmoscopy / Optical Coherence Tomography (SLO/OCT) mice retinal imaging system, as well as data acquisition and processing schemes, will be presented. Experimental results will be discussed in the context of improved morphological and functional probing of mice retinas at cellular resolution. Finally, current limitations and future directions of instrument development with a focus on improved probing of the retinal function will be discussed as well.

Original languageEnglish (US)
Title of host publicationEuropean Conference on Biomedical Optics, ECBO_2019
PublisherOSA - The Optical Society
ISBN (Electronic)9781557528209
StatePublished - Jan 1 2019
EventEuropean Conference on Biomedical Optics, ECBO_2019 - Munich, Netherlands
Duration: Jun 23 2019Jun 25 2019

Publication series

NameOptics InfoBase Conference Papers
VolumePart F142-ECBO 2019


ConferenceEuropean Conference on Biomedical Optics, ECBO_2019


  • Animal models
  • Functional retinal imaging
  • Imaging system
  • Medical optics instrumentation
  • Ophthalmology
  • Optical coherence tomography
  • Scanning Laser Ophthalmoscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials


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