Programmed bending reveals dynamic mechanochemical coupling in supported lipid bilayers

Sean F. Gilmore, Harika Nanduri, Atul N. Parikh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In living cells, mechanochemical coupling represents a dynamic means by which membrane components are spatially organized. An extra-ordinary example of such coupling involves curvature-dependent polar localization of chemically-distinct lipid domains at bacterial poles, which also undergo dramatic reequilibration upon subtle changes in their interfacial environment such as during sporulation. Here, we demonstrate that such interfacially-triggered mechanochemical coupling can be recapitulated in vitro by simultaneous, real-time introduction of mechanically-generated periodic curvatures and attendant strain-induced lateral forces in lipid bilayers supported on elastomeric substrates. In particular, we show that real-time wrinkling of the elastomeric substrate prompts a dynamic domain reorganization within the adhering bilayer, producing large, oriented liquid-ordered domains in regions of low curvature. Our results suggest a mechanism in which interfacial forces generated during surface wrinkling and the topographical deformation of the bilayer combine to facilitate dynamic reequilibration prompting the observed domain reorganization. We anticipate this curvature-generating model system will prove to be a simple and versatile tool for a broad range of studies of curvature-dependent dynamic reorganizations in membranes that are constrained by the interfacial elastic and dynamic frameworks such as the cell wall, glycocalyx, and cytoskeleton.

Original languageEnglish (US)
Article numbere28517
JournalPLoS One
Volume6
Issue number12
DOIs
StatePublished - Dec 22 2011

Fingerprint

lipid bilayers
Lipid bilayers
Lipid Bilayers
Glycocalyx
Surface Tension
Membranes
Cytoskeleton
Cell Wall
Lipids
Cells
surface tension
Substrates
cytoskeleton
sporulation
Poles
cell walls
elastomeric
liquids
Liquids
lipids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Programmed bending reveals dynamic mechanochemical coupling in supported lipid bilayers. / Gilmore, Sean F.; Nanduri, Harika; Parikh, Atul N.

In: PLoS One, Vol. 6, No. 12, e28517, 22.12.2011.

Research output: Contribution to journalArticle

Gilmore, Sean F. ; Nanduri, Harika ; Parikh, Atul N. / Programmed bending reveals dynamic mechanochemical coupling in supported lipid bilayers. In: PLoS One. 2011 ; Vol. 6, No. 12.
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