Progesterone implants enhance SIV vaginal transmission and early virus load

Preston A. Marx, Alexander I. Spira, Agegnehu Gettie, Peter J. Dailey, Ronald S. Veazey, Andrew A. Lackner, C. James Mahoney, Chris J Miller, Lee E. Claypool, David D. Ho, Nancy J. Alexander

Research output: Contribution to journalArticle

380 Citations (Scopus)

Abstract

Simian immunodeficiency virus (SIV) can cross the intact vaginal epithelium to establish a systemic infection in macaques (mac). Using this SIVmac model, we found that subcutaneous progesterone implants, which could mimic hormonally based contraceptives, thinned the vaginal epithelium and enhanced SIV vaginal transmission 7.7-fold over that observed in macaques treated with placebo implants and exposed to SIV in the follicular phase of the menstrual cycle. Progesterone treatment also increased the number of SIV DNA-positive cells in the vaginal lamina propria as detected by in situ polymerase chain reaction analysis. Moreover, plasma viral RNA was elevated for the first three months in macaques with progesterone implants, and three of the progesterone-treated macaques developed relatively rapid disease courses. This study shows that SIV genital infection and disease course are enhanced by subcutaneous implants containing progesterone when compared with the rate of vaginal transmission in the follicular phase.

Original languageEnglish (US)
Pages (from-to)1084-1089
Number of pages6
JournalNature Medicine
Volume2
Issue number10
DOIs
StatePublished - Oct 1996

Fingerprint

Simian Immunodeficiency Virus
Viruses
Progesterone
Macaca
Follicular Phase
Virus Diseases
Epithelium
Polymerase chain reaction
Viral RNA
Contraceptive Agents
Mucous Membrane
Placebos
Cells
Plasmas
Polymerase Chain Reaction
DNA
Infection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Marx, P. A., Spira, A. I., Gettie, A., Dailey, P. J., Veazey, R. S., Lackner, A. A., ... Alexander, N. J. (1996). Progesterone implants enhance SIV vaginal transmission and early virus load. Nature Medicine, 2(10), 1084-1089. https://doi.org/10.1038/nm1096-1084

Progesterone implants enhance SIV vaginal transmission and early virus load. / Marx, Preston A.; Spira, Alexander I.; Gettie, Agegnehu; Dailey, Peter J.; Veazey, Ronald S.; Lackner, Andrew A.; Mahoney, C. James; Miller, Chris J; Claypool, Lee E.; Ho, David D.; Alexander, Nancy J.

In: Nature Medicine, Vol. 2, No. 10, 10.1996, p. 1084-1089.

Research output: Contribution to journalArticle

Marx, PA, Spira, AI, Gettie, A, Dailey, PJ, Veazey, RS, Lackner, AA, Mahoney, CJ, Miller, CJ, Claypool, LE, Ho, DD & Alexander, NJ 1996, 'Progesterone implants enhance SIV vaginal transmission and early virus load', Nature Medicine, vol. 2, no. 10, pp. 1084-1089. https://doi.org/10.1038/nm1096-1084
Marx PA, Spira AI, Gettie A, Dailey PJ, Veazey RS, Lackner AA et al. Progesterone implants enhance SIV vaginal transmission and early virus load. Nature Medicine. 1996 Oct;2(10):1084-1089. https://doi.org/10.1038/nm1096-1084
Marx, Preston A. ; Spira, Alexander I. ; Gettie, Agegnehu ; Dailey, Peter J. ; Veazey, Ronald S. ; Lackner, Andrew A. ; Mahoney, C. James ; Miller, Chris J ; Claypool, Lee E. ; Ho, David D. ; Alexander, Nancy J. / Progesterone implants enhance SIV vaginal transmission and early virus load. In: Nature Medicine. 1996 ; Vol. 2, No. 10. pp. 1084-1089.
@article{65b4b14a66004f9f8d7578e2f2a0e6ef,
title = "Progesterone implants enhance SIV vaginal transmission and early virus load",
abstract = "Simian immunodeficiency virus (SIV) can cross the intact vaginal epithelium to establish a systemic infection in macaques (mac). Using this SIVmac model, we found that subcutaneous progesterone implants, which could mimic hormonally based contraceptives, thinned the vaginal epithelium and enhanced SIV vaginal transmission 7.7-fold over that observed in macaques treated with placebo implants and exposed to SIV in the follicular phase of the menstrual cycle. Progesterone treatment also increased the number of SIV DNA-positive cells in the vaginal lamina propria as detected by in situ polymerase chain reaction analysis. Moreover, plasma viral RNA was elevated for the first three months in macaques with progesterone implants, and three of the progesterone-treated macaques developed relatively rapid disease courses. This study shows that SIV genital infection and disease course are enhanced by subcutaneous implants containing progesterone when compared with the rate of vaginal transmission in the follicular phase.",
author = "Marx, {Preston A.} and Spira, {Alexander I.} and Agegnehu Gettie and Dailey, {Peter J.} and Veazey, {Ronald S.} and Lackner, {Andrew A.} and Mahoney, {C. James} and Miller, {Chris J} and Claypool, {Lee E.} and Ho, {David D.} and Alexander, {Nancy J.}",
year = "1996",
month = "10",
doi = "10.1038/nm1096-1084",
language = "English (US)",
volume = "2",
pages = "1084--1089",
journal = "Nature Medicine",
issn = "1078-8956",
publisher = "Nature Publishing Group",
number = "10",

}

TY - JOUR

T1 - Progesterone implants enhance SIV vaginal transmission and early virus load

AU - Marx, Preston A.

AU - Spira, Alexander I.

AU - Gettie, Agegnehu

AU - Dailey, Peter J.

AU - Veazey, Ronald S.

AU - Lackner, Andrew A.

AU - Mahoney, C. James

AU - Miller, Chris J

AU - Claypool, Lee E.

AU - Ho, David D.

AU - Alexander, Nancy J.

PY - 1996/10

Y1 - 1996/10

N2 - Simian immunodeficiency virus (SIV) can cross the intact vaginal epithelium to establish a systemic infection in macaques (mac). Using this SIVmac model, we found that subcutaneous progesterone implants, which could mimic hormonally based contraceptives, thinned the vaginal epithelium and enhanced SIV vaginal transmission 7.7-fold over that observed in macaques treated with placebo implants and exposed to SIV in the follicular phase of the menstrual cycle. Progesterone treatment also increased the number of SIV DNA-positive cells in the vaginal lamina propria as detected by in situ polymerase chain reaction analysis. Moreover, plasma viral RNA was elevated for the first three months in macaques with progesterone implants, and three of the progesterone-treated macaques developed relatively rapid disease courses. This study shows that SIV genital infection and disease course are enhanced by subcutaneous implants containing progesterone when compared with the rate of vaginal transmission in the follicular phase.

AB - Simian immunodeficiency virus (SIV) can cross the intact vaginal epithelium to establish a systemic infection in macaques (mac). Using this SIVmac model, we found that subcutaneous progesterone implants, which could mimic hormonally based contraceptives, thinned the vaginal epithelium and enhanced SIV vaginal transmission 7.7-fold over that observed in macaques treated with placebo implants and exposed to SIV in the follicular phase of the menstrual cycle. Progesterone treatment also increased the number of SIV DNA-positive cells in the vaginal lamina propria as detected by in situ polymerase chain reaction analysis. Moreover, plasma viral RNA was elevated for the first three months in macaques with progesterone implants, and three of the progesterone-treated macaques developed relatively rapid disease courses. This study shows that SIV genital infection and disease course are enhanced by subcutaneous implants containing progesterone when compared with the rate of vaginal transmission in the follicular phase.

UR - http://www.scopus.com/inward/record.url?scp=0345360806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345360806&partnerID=8YFLogxK

U2 - 10.1038/nm1096-1084

DO - 10.1038/nm1096-1084

M3 - Article

C2 - 8837605

AN - SCOPUS:0345360806

VL - 2

SP - 1084

EP - 1089

JO - Nature Medicine

JF - Nature Medicine

SN - 1078-8956

IS - 10

ER -