Abstract
Once a permeability barrier is overcome, proflavin is highly mutagenic for Salmonella typhimurium and Escherichia coli. Mutagenesis of the his operon (in Salmonella) and lac operon (in E. coli) depends on derepression of the respective operons. No proflavin mutagenesis was detected in rec- strains. Over 100 proflavin-induced his mutants have been classified: 50% are base substitution types, 30% are stable (10% demonstrably multisite), and only 20% are probably frameshift mutations. None of the proflavin-induced frameshift mutations is of the type previously shown to be suppressed by frameshift suppressor mutations.
| Original language | English (US) |
|---|---|
| Journal | Journal of Molecular Biology |
| Volume | 89 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 15 1974 |
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ASJC Scopus subject areas
- Virology
Cite this
Proflavin mutagenesis of bacteria. / Kohno, Tadahiko; Roth, John R.
In: Journal of Molecular Biology, Vol. 89, No. 1, 15.10.1974.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proflavin mutagenesis of bacteria
AU - Kohno, Tadahiko
AU - Roth, John R.
PY - 1974/10/15
Y1 - 1974/10/15
N2 - Once a permeability barrier is overcome, proflavin is highly mutagenic for Salmonella typhimurium and Escherichia coli. Mutagenesis of the his operon (in Salmonella) and lac operon (in E. coli) depends on derepression of the respective operons. No proflavin mutagenesis was detected in rec- strains. Over 100 proflavin-induced his mutants have been classified: 50% are base substitution types, 30% are stable (10% demonstrably multisite), and only 20% are probably frameshift mutations. None of the proflavin-induced frameshift mutations is of the type previously shown to be suppressed by frameshift suppressor mutations.
AB - Once a permeability barrier is overcome, proflavin is highly mutagenic for Salmonella typhimurium and Escherichia coli. Mutagenesis of the his operon (in Salmonella) and lac operon (in E. coli) depends on derepression of the respective operons. No proflavin mutagenesis was detected in rec- strains. Over 100 proflavin-induced his mutants have been classified: 50% are base substitution types, 30% are stable (10% demonstrably multisite), and only 20% are probably frameshift mutations. None of the proflavin-induced frameshift mutations is of the type previously shown to be suppressed by frameshift suppressor mutations.
UR - http://www.scopus.com/inward/record.url?scp=0016278325&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016278325&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(74)90160-0
DO - 10.1016/0022-2836(74)90160-0
M3 - Article
C2 - 4613853
AN - SCOPUS:0016278325
VL - 89
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 1
ER -