Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition

Sujata Halder, Susan Cotmore, Jamie Heimburg-Molinaro, David F. Smith, Richard D. Cummings, Xi Chen, Alana J. Trollope, Simon J. North, Stuart M. Haslam, Anne Dell, Peter Tattersall, Robert McKenna, Mavis Agbandje-McKenna

Research output: Contribution to journalArticle

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Abstract

The recognition of sialic acids by two strains of minute virus of mice (MVM), MVMp (prototype) and MVMi (immunosuppressive), is an essential requirement for successful infection. To understand the potential for recognition of different modifications of sialic acid by MVM, three types of capsids, virus-like particles, wild type empty (no DNA) capsids, and DNA packaged virions, were screened on a sialylated glycan microarray (SGM). Both viruses demonstrated a preference for binding to 9-O-methylated sialic acid derivatives, while MVMp showed additional binding to 9-O-acetylated and 9-O-lactoylated sialic acid derivatives, indicating recognition differences. The glycans recognized contained a type-2 Galβ1-4GlcNAc motif (Neu5Acα2-3Galβ1-4GlcNAc or 3′SIA-LN) and were biantennary complex-type N-glycans with the exception of one. To correlate the recognition of the 3′SIA-LN glycan motif as well as the biantennary structures to their natural expression in cell lines permissive for MVMp, MVMi, or both strains, the N- and O-glycans, and polar glycolipids present in three cell lines used for in vitro studies, A9 fibroblasts, EL4 T lymphocytes, and the SV40 transformed NB324K cells, were analyzed by MALDI-TOF/TOF mass spectrometry. The cells showed an abundance of the sialylated glycan motifs recognized by the viruses in the SGM and previous glycan microarrays supporting their role in cellular recognition by MVM. Significantly, the NB324K showed fucosylation at the non-reducing end of their biantennary glycans, suggesting that recognition of these cells is possibly mediated by the Lewis X motif as in 3′SIA-LeX identified in a previous glycan microarray screen.

Original languageEnglish (US)
Article numbere86909
JournalPLoS One
Volume9
Issue number1
DOIs
StatePublished - Jan 27 2014

Fingerprint

Minute Virus of Mice
Viruses
Polysaccharides
polysaccharides
Cells
cell lines
Cell Line
receptors
sialic acids
Microarrays
cells
N-Acetylneuraminic Acid
capsid
Capsid
Virion
Rodent protoparvovirus 1
Sialic Acids
Derivatives
immunosuppressive agents
viruses

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Halder, S., Cotmore, S., Heimburg-Molinaro, J., Smith, D. F., Cummings, R. D., Chen, X., ... Agbandje-McKenna, M. (2014). Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition. PLoS One, 9(1), [e86909]. https://doi.org/10.1371/journal.pone.0086909

Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition. / Halder, Sujata; Cotmore, Susan; Heimburg-Molinaro, Jamie; Smith, David F.; Cummings, Richard D.; Chen, Xi; Trollope, Alana J.; North, Simon J.; Haslam, Stuart M.; Dell, Anne; Tattersall, Peter; McKenna, Robert; Agbandje-McKenna, Mavis.

In: PLoS One, Vol. 9, No. 1, e86909, 27.01.2014.

Research output: Contribution to journalArticle

Halder, S, Cotmore, S, Heimburg-Molinaro, J, Smith, DF, Cummings, RD, Chen, X, Trollope, AJ, North, SJ, Haslam, SM, Dell, A, Tattersall, P, McKenna, R & Agbandje-McKenna, M 2014, 'Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition', PLoS One, vol. 9, no. 1, e86909. https://doi.org/10.1371/journal.pone.0086909
Halder, Sujata ; Cotmore, Susan ; Heimburg-Molinaro, Jamie ; Smith, David F. ; Cummings, Richard D. ; Chen, Xi ; Trollope, Alana J. ; North, Simon J. ; Haslam, Stuart M. ; Dell, Anne ; Tattersall, Peter ; McKenna, Robert ; Agbandje-McKenna, Mavis. / Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition. In: PLoS One. 2014 ; Vol. 9, No. 1.
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