Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection

Jeremy J W Chen, Reen Wu, Pan Chyr Yang, Jane Yu Huang, Yuh Pyng Sher, Meng Hsuan Han, Wei Chen Kao, Pei Jung Lee, Trai Fu Chiu, Fu Chang, Yi Wen Chu, Cheng Wen Wu, Konan Peck

Research output: Contribution to journalArticle

195 Scopus citations

Abstract

A high-density cDNA microarray with colorimetry detection system to simultaneously monitor the expression of many genes on nylon membrane is described and characterized. To quantify the expression of genes and to isolate differentially expressed genes, the southern hybridization process on filter membranes was employed. The levels of gene expression were represented by color intensities generated by colorimetric reactions in place of hazardous radioisotopes or costly laser-induced fluorescence detection. The gene expression patterns on nylon membranes were digitized by devices such as an economical flatbed scanner or a digital camera. The quantitative information of gene expression was retrieved by image analysis software. Quantitative comparison of the northern dot-blotting method with the microarray system is described. Applications employing single-color detection as well as dual-color detection to isolate differentially expressed genes among thousands of genes are demonstrated.

Original languageEnglish (US)
Pages (from-to)313-324
Number of pages12
JournalGenomics
Volume51
Issue number3
DOIs
StatePublished - Aug 1 1998
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics

Cite this

Chen, J. J. W., Wu, R., Yang, P. C., Huang, J. Y., Sher, Y. P., Han, M. H., Kao, W. C., Lee, P. J., Chiu, T. F., Chang, F., Chu, Y. W., Wu, C. W., & Peck, K. (1998). Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection. Genomics, 51(3), 313-324. https://doi.org/10.1006/geno.1998.5354