Production of trihydroxy derivatives of arachidonic and docosahexaenoic acid by lipoxygenase activity in trout gill tissue

J. Brace German, John E. Kinsella

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The production of polyoxygenated derivatives of arachidonic acid as a consequence of nonenzymatic peroxidation of the immediate product of lipoxygenase activity occurs in animal tissues. This investigation was conducted to determine if n-3 docosahexaenoic acid was susceptible to similar reactions. Metabolism of arachidonic acid (20:4(n-6)) and docosahexaenoic acid (22:6(n-3)) by the 12-lipoxygenase of trout gill was studied in a model system. Results showed that high levels of lipoxygenase activity led to the proliferation of comparable products from both fatty acids. Analyses of these compounds by GC-MS identified in addition to the 12- and 14-monohydroxy derivatives, respectively, mixtures of the 8,11,12- and 10,11,12-trihydroxy products of 20:4 and the 10,13,14- and 12,13,14-trihydroxy derivatives of 22:6.

Original languageEnglish (US)
Pages (from-to)290-298
Number of pages9
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Issue number2
StatePublished - Jun 27 1986
Externally publishedYes



  • Arachidonic acid
  • Arachidonic acid metabolism
  • Docosahexaenoic acid
  • Lipoxygenase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology

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