Production of stable-isotope-labeled bovine heme and its use to measure heme-iron absorption in children

Paz Etcheverry, Gordon E. Carstens, Erin Brown, Keli M. Hawthorne, Zhensheng Chen, Ian J. Griffin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: The use of stable isotopes has provided valuable insights into iron absorption in humans, but the data have been limited to nonheme iron. Objective: Our objectives were to produce heme iron enriched in 58Fe and to use it to study the absorption of heme iron and the effect of iron and zinc intakes on heme-iron absorption in children. Design: Labeled bovine heme was produced in a bovine model. Forty-eight children were randomly assigned to consume identical meals containing 1 of 3 doses of labeled heme iron (2, 4, or 8 mg as hemoglobin) and 1 of 2 doses of inorganic zinc (1 or 9 mg); successful measurements of iron absorption, zinc absorption, or both were made in 40 of these subjects. We hypothesized that fractional heme-iron absorption would decrease as heme-iron intake increased and that higher zinc intakes would decrease heme-iron absorption. Results: 58Fe heme was produced with an enrichment (mass/mass) of 9.5%. Fractional iron absorption in children was significantly affected by the intake of heme iron (P = 0.0013) and of zinc (P = 0.0375), but, contrary to expectations, heme-iron absorption was higher at higher zinc intakes. Absolute heme-iron absorption was higher in the group with higher zinc intakes, but only for those with the lowest heme-iron intake (2 mg;P = 0.0147). Although fractional zinc absorption decreased as zinc intake increased (P = 0.031), absolute zinc absorption continued to increase across the intake range studied (P = 0.018). Conclusions: Heme iron intrinsically labeled with 58Fe can be produced at sufficient enrichments for use in human studies. In children, heme iron and zinc absorption decrease as the dose of each mineral increases. Heme iron did not inhibit zinc absorption. At lower heme intakes, zinc intakes may increase heme-iron absorption.

Original languageEnglish (US)
Pages (from-to)452-459
Number of pages8
JournalAmerican Journal of Clinical Nutrition
Volume85
Issue number2
StatePublished - Feb 1 2007
Externally publishedYes

Fingerprint

heme iron
iron absorption
heme
Heme
Isotopes
stable isotopes
Iron
zinc
Zinc
cattle
dosage
iron

Keywords

  • Children
  • Heme iron
  • Iron absorption
  • Stable isotope
  • Zinc absorption

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Etcheverry, P., Carstens, G. E., Brown, E., Hawthorne, K. M., Chen, Z., & Griffin, I. J. (2007). Production of stable-isotope-labeled bovine heme and its use to measure heme-iron absorption in children. American Journal of Clinical Nutrition, 85(2), 452-459.

Production of stable-isotope-labeled bovine heme and its use to measure heme-iron absorption in children. / Etcheverry, Paz; Carstens, Gordon E.; Brown, Erin; Hawthorne, Keli M.; Chen, Zhensheng; Griffin, Ian J.

In: American Journal of Clinical Nutrition, Vol. 85, No. 2, 01.02.2007, p. 452-459.

Research output: Contribution to journalArticle

Etcheverry, P, Carstens, GE, Brown, E, Hawthorne, KM, Chen, Z & Griffin, IJ 2007, 'Production of stable-isotope-labeled bovine heme and its use to measure heme-iron absorption in children', American Journal of Clinical Nutrition, vol. 85, no. 2, pp. 452-459.
Etcheverry P, Carstens GE, Brown E, Hawthorne KM, Chen Z, Griffin IJ. Production of stable-isotope-labeled bovine heme and its use to measure heme-iron absorption in children. American Journal of Clinical Nutrition. 2007 Feb 1;85(2):452-459.
Etcheverry, Paz ; Carstens, Gordon E. ; Brown, Erin ; Hawthorne, Keli M. ; Chen, Zhensheng ; Griffin, Ian J. / Production of stable-isotope-labeled bovine heme and its use to measure heme-iron absorption in children. In: American Journal of Clinical Nutrition. 2007 ; Vol. 85, No. 2. pp. 452-459.
@article{404eb55bdecc405898bb44d43ae101a5,
title = "Production of stable-isotope-labeled bovine heme and its use to measure heme-iron absorption in children",
abstract = "Background: The use of stable isotopes has provided valuable insights into iron absorption in humans, but the data have been limited to nonheme iron. Objective: Our objectives were to produce heme iron enriched in 58Fe and to use it to study the absorption of heme iron and the effect of iron and zinc intakes on heme-iron absorption in children. Design: Labeled bovine heme was produced in a bovine model. Forty-eight children were randomly assigned to consume identical meals containing 1 of 3 doses of labeled heme iron (2, 4, or 8 mg as hemoglobin) and 1 of 2 doses of inorganic zinc (1 or 9 mg); successful measurements of iron absorption, zinc absorption, or both were made in 40 of these subjects. We hypothesized that fractional heme-iron absorption would decrease as heme-iron intake increased and that higher zinc intakes would decrease heme-iron absorption. Results: 58Fe heme was produced with an enrichment (mass/mass) of 9.5{\%}. Fractional iron absorption in children was significantly affected by the intake of heme iron (P = 0.0013) and of zinc (P = 0.0375), but, contrary to expectations, heme-iron absorption was higher at higher zinc intakes. Absolute heme-iron absorption was higher in the group with higher zinc intakes, but only for those with the lowest heme-iron intake (2 mg;P = 0.0147). Although fractional zinc absorption decreased as zinc intake increased (P = 0.031), absolute zinc absorption continued to increase across the intake range studied (P = 0.018). Conclusions: Heme iron intrinsically labeled with 58Fe can be produced at sufficient enrichments for use in human studies. In children, heme iron and zinc absorption decrease as the dose of each mineral increases. Heme iron did not inhibit zinc absorption. At lower heme intakes, zinc intakes may increase heme-iron absorption.",
keywords = "Children, Heme iron, Iron absorption, Stable isotope, Zinc absorption",
author = "Paz Etcheverry and Carstens, {Gordon E.} and Erin Brown and Hawthorne, {Keli M.} and Zhensheng Chen and Griffin, {Ian J.}",
year = "2007",
month = "2",
day = "1",
language = "English (US)",
volume = "85",
pages = "452--459",
journal = "American Journal of Clinical Nutrition",
issn = "0002-9165",
publisher = "American Society for Nutrition",
number = "2",

}

TY - JOUR

T1 - Production of stable-isotope-labeled bovine heme and its use to measure heme-iron absorption in children

AU - Etcheverry, Paz

AU - Carstens, Gordon E.

AU - Brown, Erin

AU - Hawthorne, Keli M.

AU - Chen, Zhensheng

AU - Griffin, Ian J.

PY - 2007/2/1

Y1 - 2007/2/1

N2 - Background: The use of stable isotopes has provided valuable insights into iron absorption in humans, but the data have been limited to nonheme iron. Objective: Our objectives were to produce heme iron enriched in 58Fe and to use it to study the absorption of heme iron and the effect of iron and zinc intakes on heme-iron absorption in children. Design: Labeled bovine heme was produced in a bovine model. Forty-eight children were randomly assigned to consume identical meals containing 1 of 3 doses of labeled heme iron (2, 4, or 8 mg as hemoglobin) and 1 of 2 doses of inorganic zinc (1 or 9 mg); successful measurements of iron absorption, zinc absorption, or both were made in 40 of these subjects. We hypothesized that fractional heme-iron absorption would decrease as heme-iron intake increased and that higher zinc intakes would decrease heme-iron absorption. Results: 58Fe heme was produced with an enrichment (mass/mass) of 9.5%. Fractional iron absorption in children was significantly affected by the intake of heme iron (P = 0.0013) and of zinc (P = 0.0375), but, contrary to expectations, heme-iron absorption was higher at higher zinc intakes. Absolute heme-iron absorption was higher in the group with higher zinc intakes, but only for those with the lowest heme-iron intake (2 mg;P = 0.0147). Although fractional zinc absorption decreased as zinc intake increased (P = 0.031), absolute zinc absorption continued to increase across the intake range studied (P = 0.018). Conclusions: Heme iron intrinsically labeled with 58Fe can be produced at sufficient enrichments for use in human studies. In children, heme iron and zinc absorption decrease as the dose of each mineral increases. Heme iron did not inhibit zinc absorption. At lower heme intakes, zinc intakes may increase heme-iron absorption.

AB - Background: The use of stable isotopes has provided valuable insights into iron absorption in humans, but the data have been limited to nonheme iron. Objective: Our objectives were to produce heme iron enriched in 58Fe and to use it to study the absorption of heme iron and the effect of iron and zinc intakes on heme-iron absorption in children. Design: Labeled bovine heme was produced in a bovine model. Forty-eight children were randomly assigned to consume identical meals containing 1 of 3 doses of labeled heme iron (2, 4, or 8 mg as hemoglobin) and 1 of 2 doses of inorganic zinc (1 or 9 mg); successful measurements of iron absorption, zinc absorption, or both were made in 40 of these subjects. We hypothesized that fractional heme-iron absorption would decrease as heme-iron intake increased and that higher zinc intakes would decrease heme-iron absorption. Results: 58Fe heme was produced with an enrichment (mass/mass) of 9.5%. Fractional iron absorption in children was significantly affected by the intake of heme iron (P = 0.0013) and of zinc (P = 0.0375), but, contrary to expectations, heme-iron absorption was higher at higher zinc intakes. Absolute heme-iron absorption was higher in the group with higher zinc intakes, but only for those with the lowest heme-iron intake (2 mg;P = 0.0147). Although fractional zinc absorption decreased as zinc intake increased (P = 0.031), absolute zinc absorption continued to increase across the intake range studied (P = 0.018). Conclusions: Heme iron intrinsically labeled with 58Fe can be produced at sufficient enrichments for use in human studies. In children, heme iron and zinc absorption decrease as the dose of each mineral increases. Heme iron did not inhibit zinc absorption. At lower heme intakes, zinc intakes may increase heme-iron absorption.

KW - Children

KW - Heme iron

KW - Iron absorption

KW - Stable isotope

KW - Zinc absorption

UR - http://www.scopus.com/inward/record.url?scp=33846886115&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846886115&partnerID=8YFLogxK

M3 - Article

VL - 85

SP - 452

EP - 459

JO - American Journal of Clinical Nutrition

JF - American Journal of Clinical Nutrition

SN - 0002-9165

IS - 2

ER -