Production of pseudotyped retrovirus and the generation of proviral transgenic zebrafish.

Li-En Jao, Shawn M. Burgess

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This chapter describes a method for generation of the high-titer pseudotyped Moloney murine leukemia virus (MLV) that efficiently infects zebrafish embryos (i.e., more than 25 retroviral copies per cell). Injection techniques are also described for production of the retrovirus-infected mosaic "founder" fish. We describe a quantitative PCR (qPCR)-based assay as a quick way to assess the infectivity after each round of viral production and injection. Most of the required equipment is commercially available and commonly present in most research laboratories.

Original languageEnglish (US)
Pages (from-to)13-30
Number of pages18
JournalMethods in molecular biology (Clifton, N.J.)
Volume546
DOIs
StatePublished - 2009
Externally publishedYes

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Zebrafish
Retroviridae
Moloney murine leukemia virus
Injections
Fishes
Embryonic Structures
Equipment and Supplies
Polymerase Chain Reaction
Research

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Production of pseudotyped retrovirus and the generation of proviral transgenic zebrafish. / Jao, Li-En; Burgess, Shawn M.

In: Methods in molecular biology (Clifton, N.J.), Vol. 546, 2009, p. 13-30.

Research output: Contribution to journalArticle

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