Production of prostaglandins E2 and F2(α) in the freshwater mussel Ligumia subrostrata: Relation to sodium transport

D. G. Saintsing, D. H. Hwang, T. H. Dietz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Pharmacological experiments indicate that prostaglandins (PGs) have a role in the control of sodium regulation in freshwater mussels and the mechanism may be linked to cyclic AMP and serotonin. To test this hypothesis we used radioimmunoassay to investigate the ability of freshwater mussels to synthesize PGs. The levels of precursor fatty acids were determined in a gas-liquid chromatograph. Arachidonic acid (precursor to the diene PGs) was the major fatty acid component of total lipids in the gill and accounted for 14% of the total. In addition, gill homogenates synthesize PG-like material from [3H]arachidonic acid. Material corresponding to PGE2 and PGF2(α) were identified on thin-layer radiochromatograms. These data indicate that gills (the primary site of Na transport) can produce PGs. The presence of PGs in freshwater mussels was verified by radioimmunoassay of blood. Both PGE2 and PGF2(α) were identified using highly specific antisera. The concentrations of both PGs was significantly reduced when the mussels were injected with inhibitors of phospholipase A2 or cyclooxygenase before sampling blood. Stimulation of Na transport by serotonin and cyclic AMP results in a depression of blood PGE2 with no effect on circulating PGF2(α). PGE2 levels are inversely correlated with net Na flux. These data indicate endogenous PGE2 negatively modulated Na transport and PGE2 levels are regulated by a serotonin-cyclic AMP mediated system.

Original languageEnglish (US)
Pages (from-to)455-461
Number of pages7
JournalJournal of Pharmacology and Experimental Therapeutics
Volume226
Issue number2
StatePublished - 1983
Externally publishedYes

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Dinoprost
Bivalvia
Fresh Water
Dinoprostone
Prostaglandins
Sodium
Cyclic AMP
Serotonin
Arachidonic Acid
Radioimmunoassay
Fatty Acids
Phospholipase A2 Inhibitors
Prostaglandin-Endoperoxide Synthases
Immune Sera
Gases
Pharmacology
Lipids

ASJC Scopus subject areas

  • Pharmacology

Cite this

Production of prostaglandins E2 and F2(α) in the freshwater mussel Ligumia subrostrata : Relation to sodium transport. / Saintsing, D. G.; Hwang, D. H.; Dietz, T. H.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 226, No. 2, 1983, p. 455-461.

Research output: Contribution to journalArticle

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