Production of polyclonal antisera against feline immunoglobulin E and its use in an ELISA in cats with experimentally induced asthma

C. R. Norris, K. C. Decile, J. R. Byerly, R. D. Berghaus, Edward S Schelegle, D. M. Hyde, Laurel J Gershwin

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Serum samples from six cats with experimentally induced asthma were used to purify feline IgE using gel filtration and affinity chromatography. The resultant IgE, evaluated for purity by immunoelectrophoresis (IEP) and reactivity by Prausnitz-Kustner (PK) testing, was used to develop polyclonal rabbit anti-feline IgE antisera. Using reverse cutaneous anaphylaxis (RCA), the antisera were determined to be specific for feline IgE. The polyclonal rabbit anti-feline IgE antiserum was then validated in an allergen-specific ELISA. Serum samples from an additional five asthmatic cats sensitized with Bermuda grass allergen (BGA) were evaluated prior to sensitization, after parenteral sensitization, and after aerosol sensitization and challenge. A significant increase in serum BGA-specific IgE was noted over time.

Original languageEnglish (US)
Pages (from-to)149-157
Number of pages9
JournalVeterinary Immunology and Immunopathology
Volume96
Issue number3-4
DOIs
StatePublished - Dec 15 2003

Keywords

  • Allergy
  • Bermuda grass allergen
  • Cat
  • Prausnitz-Kustner test
  • Sensitization

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

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