Production of ovine chimeras by inner cell mass transplantation.

J. E. Butler, G. B. Anderson, Robert Bondurant, R. L. Pashen, Cecilia Penedo

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Ovine chimeras were produced by micro-injection of isolated inner cell masses (ICM) into recipient blastocysts. Inner cell masses were isolated by immunosurgery. A total of 57 chimeric embryos was produced, 52 of which were transferred to recipient ewes. Thirty-seven live lambs were born, of which 15 were determined to be chimeric on the basis of blood type analysis. One lamb, although not a blood chimera, exhibited overt signs of chimerism. An additional six lambs were determined to have developed solely from the injected ICM. The rate of chimerism in live lambs was 43% (16/37) while the survival rate of injected ICM was 59% (22/37). The method presented allows the production of relatively large proportion of viable, chimeric embryos without the use of an intermediate recipient.

Original languageEnglish (US)
Pages (from-to)317-324
Number of pages8
JournalJournal of Animal Science
Volume65
Issue number1
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

Fingerprint

chimerism
Cell Transplantation
Sheep
lambs
sheep
Chimerism
embryo (animal)
Embryonic Structures
cells
blood groups
Blastocyst
blastocyst
ewes
survival rate
injection
Injections
blood
methodology

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Production of ovine chimeras by inner cell mass transplantation. / Butler, J. E.; Anderson, G. B.; Bondurant, Robert; Pashen, R. L.; Penedo, Cecilia.

In: Journal of Animal Science, Vol. 65, No. 1, 01.01.1987, p. 317-324.

Research output: Contribution to journalArticle

Butler, J. E. ; Anderson, G. B. ; Bondurant, Robert ; Pashen, R. L. ; Penedo, Cecilia. / Production of ovine chimeras by inner cell mass transplantation. In: Journal of Animal Science. 1987 ; Vol. 65, No. 1. pp. 317-324.
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