Abstract
The procedure currently used for the production of Streptococcus suis antigen is very long and includes several subcultures. The aim of the present work was to study the in vitro production of capsular material by S. suis serotype 2 after each of these subcultures. The amount of capsular material produced was evaluated by electron microscopy using bacterial cells grown on blood-agar plates and in Todd-Hewitt broth (THB) or THB supplemented with serum. In addition, the production of antibodies in rabbits with antigens produced using different growth conditions was compared. Antigens produced after only three subcultures possessed as much capsular material as cells obtained after the complete procedure and induced a similar antibody response. The use of serum as a supplement to the broth did not assure a higher production of capsule; in addition, antibody titers obtained with antigens produced in THB were as high as those obtained with antigens produced in THB supplemented with serum. We recommend the use of three subcultures in nonsupplemented broth for the production of immunogens. This revised protocol offers two main advantages: it is less time-consuming because of the limited number of subcultures and is also less expensive since nonsupplemented broths are used.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 49-52 |
| Number of pages | 4 |
| Journal | Canadian journal of veterinary research = Revue canadienne de recherche veterinaire |
| Volume | 57 |
| Issue number | 1 |
| State | Published - Jan 1 1993 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- veterinary(all)
Cite this
Production of capsular material by Streptococcus suis serotype 2 under different growth conditions. / Gottschalk, M.; Higgins, Robert; Jacques, M.
In: Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, Vol. 57, No. 1, 01.01.1993, p. 49-52.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of capsular material by Streptococcus suis serotype 2 under different growth conditions.
AU - Gottschalk, M.
AU - Higgins, Robert
AU - Jacques, M.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - The procedure currently used for the production of Streptococcus suis antigen is very long and includes several subcultures. The aim of the present work was to study the in vitro production of capsular material by S. suis serotype 2 after each of these subcultures. The amount of capsular material produced was evaluated by electron microscopy using bacterial cells grown on blood-agar plates and in Todd-Hewitt broth (THB) or THB supplemented with serum. In addition, the production of antibodies in rabbits with antigens produced using different growth conditions was compared. Antigens produced after only three subcultures possessed as much capsular material as cells obtained after the complete procedure and induced a similar antibody response. The use of serum as a supplement to the broth did not assure a higher production of capsule; in addition, antibody titers obtained with antigens produced in THB were as high as those obtained with antigens produced in THB supplemented with serum. We recommend the use of three subcultures in nonsupplemented broth for the production of immunogens. This revised protocol offers two main advantages: it is less time-consuming because of the limited number of subcultures and is also less expensive since nonsupplemented broths are used.
AB - The procedure currently used for the production of Streptococcus suis antigen is very long and includes several subcultures. The aim of the present work was to study the in vitro production of capsular material by S. suis serotype 2 after each of these subcultures. The amount of capsular material produced was evaluated by electron microscopy using bacterial cells grown on blood-agar plates and in Todd-Hewitt broth (THB) or THB supplemented with serum. In addition, the production of antibodies in rabbits with antigens produced using different growth conditions was compared. Antigens produced after only three subcultures possessed as much capsular material as cells obtained after the complete procedure and induced a similar antibody response. The use of serum as a supplement to the broth did not assure a higher production of capsule; in addition, antibody titers obtained with antigens produced in THB were as high as those obtained with antigens produced in THB supplemented with serum. We recommend the use of three subcultures in nonsupplemented broth for the production of immunogens. This revised protocol offers two main advantages: it is less time-consuming because of the limited number of subcultures and is also less expensive since nonsupplemented broths are used.
UR - http://www.scopus.com/inward/record.url?scp=0027352406&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027352406&partnerID=8YFLogxK
M3 - Article
C2 - 8431805
AN - SCOPUS:0027352406
VL - 57
SP - 49
EP - 52
JO - Canadian Journal of Veterinary Research
JF - Canadian Journal of Veterinary Research
SN - 0830-9000
IS - 1
ER -