Production of a type 2 maternal diabetes rodent model using the combination of high-fat diet and moderate dose of streptozocin

Juan claudio Gutierrez, Javiera Bahamonde, M. Renee Prater, Claudia P. Yefi, Steven D. Holladay

Research output: Contribution to journalArticle

4 Scopus citations


Introduction. Pregnancy may be complicated by maternal diabetes. The following experiments were performed in an attempt to produce mouse models of insulin-resistant maternal diabetes. Methods. CD1 females received 200 mg/kg streptozocin (STZ) to model insulin-dependent diabetes (T1 group). Another group of females (T2 group) was put on a HFD 4 weeks before receiving 100 mg/kg STZ. After 4 additional weeks of HFD, hyperglycemic females were separated and bred. In another experiement, CD1 females were fed a HFD for 4 weeks before receiving an intravenous (GDM1 group) or intraperitoneal (GDM2 group) injection of 100 mg/kg STZ. Females from GDM2 group were bred at the same day of the STZ injection. Females from GDM1 group were bred 4 weeks after the STZ injection. Results and Conclusion. About 25% of the females from T2 group became hyperglycemic after 4 weeks of the injection of STZ. Fifty percent of the females from GDM1 group reached hyperglycemic levels greater than 250 mg/dl during pregnancy. The combination of HFD and moderate STZ in CD1 mice therefore produced hyperglycemic females; however numbers of these mice were somewhat low.

Original languageEnglish (US)
Pages (from-to)59-70
Number of pages12
JournalEndocrine Research
Issue number2
StatePublished - May 2010
Externally publishedYes



  • Diabetes and Pregnancy
  • Mouse Models of Diabetes
  • Type 1 Diabetes
  • Type 2 Diabetes

ASJC Scopus subject areas

  • Endocrinology

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