Abstract
Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.
Original language | English (US) |
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Pages (from-to) | 60-65 |
Number of pages | 6 |
Journal | Data in Brief |
Volume | 7 |
DOIs | |
State | Published - Jun 1 2016 |
ASJC Scopus subject areas
- General
- Education