Abstract
Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 60-65 |
| Number of pages | 6 |
| Journal | Data in Brief |
| Volume | 7 |
| DOIs | |
| State | Published - Jun 1 2016 |
ASJC Scopus subject areas
- General
- Education
Cite this
Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization. / Church, Molly E.; Dela Cruz, Florante N.; Kim, Kevin; Persiani, Michele; Woods, Leslie; Pesavento, Patricia; Woolard, Kevin D.
In: Data in Brief, Vol. 7, 01.06.2016, p. 60-65.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization
AU - Church, Molly E.
AU - Dela Cruz, Florante N.
AU - Kim, Kevin
AU - Persiani, Michele
AU - Woods, Leslie
AU - Pesavento, Patricia
AU - Woolard, Kevin D
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.
AB - Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.
UR - http://www.scopus.com/inward/record.url?scp=84958256055&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84958256055&partnerID=8YFLogxK
U2 - 10.1016/j.dib.2016.01.070
DO - 10.1016/j.dib.2016.01.070
M3 - Article
VL - 7
SP - 60
EP - 65
JO - Data in Brief
JF - Data in Brief
SN - 2352-3409
ER -