Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization

Molly E. Church, Florante N. Dela Cruz, Kevin Kim, Michele Persiani, Leslie Woods, Patricia Pesavento, Kevin D Woolard

Research output: Contribution to journalArticle

Abstract

Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.

Original languageEnglish (US)
Pages (from-to)60-65
Number of pages6
JournalData in Brief
Volume7
DOIs
StatePublished - Jun 1 2016

ASJC Scopus subject areas

  • General
  • Education

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