Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization

Molly E. Church; Florante N. Dela Cruz; Kevin Kim; Michele Persiani; Leslie Woods; Patricia Pesavento; Kevin D Woolard

doi:10.1016/j.dib.2016.01.070

Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization

Molly E. Church, Florante N. Dela Cruz, Kevin Kim, Michele Persiani, Leslie Woods, Patricia Pesavento, Kevin D Woolard

Research output: Contribution to journal › Article › peer-review

Abstract

Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.

Original language	English (US)
Pages (from-to)	60-65
Number of pages	6
Journal	Data in Brief
Volume	7
DOIs	https://doi.org/10.1016/j.dib.2016.01.070
State	Published - Jun 1 2016

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Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization. / Church, Molly E.; Dela Cruz, Florante N.; Kim, Kevin; Persiani, Michele; Woods, Leslie ; Pesavento, Patricia ; Woolard, Kevin D.

In: Data in Brief, Vol. 7, 01.06.2016, p. 60-65.

Research output: Contribution to journal › Article › peer-review

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abstract = "Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.",

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AU - Persiani, Michele

AU - Woods, Leslie

AU - Pesavento, Patricia

AU - Woolard, Kevin D

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Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization

Abstract

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