Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization

Molly E. Church; Florante N. Dela Cruz; Kevin Kim; Michele Persiani; Leslie Woods; Patricia Pesavento; Kevin D Woolard

doi:10.1016/j.dib.2016.01.070

Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization

Molly E. Church, Florante N. Dela Cruz, Kevin Kim, Michele Persiani, Leslie Woods, Patricia Pesavento, Kevin D Woolard

Research output: Contribution to journal › Article

Abstract

Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.

Original language	English (US)
Pages (from-to)	60-65
Number of pages	6
Journal	Data in Brief
Volume	7
DOIs	https://doi.org/10.1016/j.dib.2016.01.070
State	Published - Jun 1 2016

ASJC Scopus subject areas

General
Education

Cite this

Church, M. E., Dela Cruz, F. N., Kim, K., Persiani, M., Woods, L., Pesavento, P., & Woolard, K. D. (2016). Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization. Data in Brief, 7, 60-65. https://doi.org/10.1016/j.dib.2016.01.070

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10.1016/j.dib.2016.01.070