Abstract
α-Galactosyl epitopes (or α-Gal, oligosaccharides with a terminal Galα1,3Gal sequence) are a class of biologically important oligosaccharides in great demand in bulk quantities for basic and clinical studies on preventing hyperacute rejection in pig-to-primate organ xenotransplantaion. A truncated bovine α-1,3-galactosyltransferase, the key enzyme responsible for the biosynthesis of the terminal structure of α-Gal, was cloned and overexpressed previously. The acceptor specificity was further studied in the present paper, and lactose and galactose derivatives were found to be good acceptors. To develop a more proficient reaction process, we report herein an example of an efficient enzymatic synthesis of α-Gal oligosaccharides catalyzed by the combination of two recombinant Escherichia coli whole cells harboring the genes of a UDP-galactose 4-epimerase and the α-1,3-galactosyltransferase, respectively. Using lactosyl azide (LacN3) as the acceptor for the glycosyltransferase, the combined use of the two recombinant cells efficiently produced α-Gal epitope Galα1,3LacN3 in 60-68% yield.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 595-599 |
| Number of pages | 5 |
| Journal | Biotechnology Progress |
| Volume | 16 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 2000 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Food Science
- Biotechnology
- Microbiology
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Production of α-Galactosyl epitopes via combined use of two recombinant whole cells harboring UDP-galactose 4-epimerase and α-1,3-galactosyltransferase. / Chen, Xi; Zhang, Wei; Wang, Jianqiang; Fang, Jianwen; Wang, Peng George.
In: Biotechnology Progress, Vol. 16, No. 4, 07.2000, p. 595-599.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Production of α-Galactosyl epitopes via combined use of two recombinant whole cells harboring UDP-galactose 4-epimerase and α-1,3-galactosyltransferase
AU - Chen, Xi
AU - Zhang, Wei
AU - Wang, Jianqiang
AU - Fang, Jianwen
AU - Wang, Peng George
PY - 2000/7
Y1 - 2000/7
N2 - α-Galactosyl epitopes (or α-Gal, oligosaccharides with a terminal Galα1,3Gal sequence) are a class of biologically important oligosaccharides in great demand in bulk quantities for basic and clinical studies on preventing hyperacute rejection in pig-to-primate organ xenotransplantaion. A truncated bovine α-1,3-galactosyltransferase, the key enzyme responsible for the biosynthesis of the terminal structure of α-Gal, was cloned and overexpressed previously. The acceptor specificity was further studied in the present paper, and lactose and galactose derivatives were found to be good acceptors. To develop a more proficient reaction process, we report herein an example of an efficient enzymatic synthesis of α-Gal oligosaccharides catalyzed by the combination of two recombinant Escherichia coli whole cells harboring the genes of a UDP-galactose 4-epimerase and the α-1,3-galactosyltransferase, respectively. Using lactosyl azide (LacN3) as the acceptor for the glycosyltransferase, the combined use of the two recombinant cells efficiently produced α-Gal epitope Galα1,3LacN3 in 60-68% yield.
AB - α-Galactosyl epitopes (or α-Gal, oligosaccharides with a terminal Galα1,3Gal sequence) are a class of biologically important oligosaccharides in great demand in bulk quantities for basic and clinical studies on preventing hyperacute rejection in pig-to-primate organ xenotransplantaion. A truncated bovine α-1,3-galactosyltransferase, the key enzyme responsible for the biosynthesis of the terminal structure of α-Gal, was cloned and overexpressed previously. The acceptor specificity was further studied in the present paper, and lactose and galactose derivatives were found to be good acceptors. To develop a more proficient reaction process, we report herein an example of an efficient enzymatic synthesis of α-Gal oligosaccharides catalyzed by the combination of two recombinant Escherichia coli whole cells harboring the genes of a UDP-galactose 4-epimerase and the α-1,3-galactosyltransferase, respectively. Using lactosyl azide (LacN3) as the acceptor for the glycosyltransferase, the combined use of the two recombinant cells efficiently produced α-Gal epitope Galα1,3LacN3 in 60-68% yield.
UR - http://www.scopus.com/inward/record.url?scp=0034233443&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034233443&partnerID=8YFLogxK
U2 - 10.1021/bp000052s
DO - 10.1021/bp000052s
M3 - Article
C2 - 10933834
AN - SCOPUS:0034233443
VL - 16
SP - 595
EP - 599
JO - Biotechnology Progress
JF - Biotechnology Progress
SN - 8756-7938
IS - 4
ER -