Production and transplantation of retinal cells from human and mouse embryonic stem cells

Anna La Torre Vila, Deepak A. Lamba, Anu Jayabalu, Thomas A. Reh

Research output: Chapter in Book/Report/Conference proceedingChapter

22 Scopus citations

Abstract

Over the last few years, numerous studies have introduced strategies for the generation of neuronal populations from embryonic stem cells. These techniques are valuable both in the study of early neurogenesis and in the generation of an unlimited source of donor cells for replacement therapies. We have developed a protocol to direct mouse and human embryonic stem cells to retinal fates by using the current model of eye specification. Our method is a multistep protocol in which the cultures are treated with IGF1 and a combination of BMP and Wnt inhibitors to promote the expression of key retinal progenitor genes, as assayed by RT-PCR and immunofluorescence microscopy. The retinal progenitor population spontaneously undergoes differentiation towards various types of retinal neurons, including photoreceptors.

Original languageEnglish (US)
Title of host publicationRetinal Development: Methods and Protocols
PublisherHumana Press Inc.
Pages229-246
Number of pages18
Volume884
ISBN (Print)9781617798474
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume884
ISSN (Print)10643745

Keywords

  • Eye field
  • hES cells
  • mES cells
  • Photoreceptor
  • Retinal progenitor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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  • Cite this

    La Torre Vila, A., Lamba, D. A., Jayabalu, A., & Reh, T. A. (2012). Production and transplantation of retinal cells from human and mouse embryonic stem cells. In Retinal Development: Methods and Protocols (Vol. 884, pp. 229-246). (Methods in Molecular Biology; Vol. 884). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-848-1_16