Processing of wild caught Culicoides and isolation of bluetongue virus using a mosquito cell line

Mary Sawyer, Bennie Osburn

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Isolation of bluetongue virus from the Culicoides vector can provide valuable epidemiologic information. The methods described offer a practical procedure that ensures the preservation of the virus during field processing of the collection and an assay system that utilizes C6/36 cells, a clone derived from Aedes albopictus. The C6/36 cell line affords a sensitive isolation system and a practical virus detection system suitable for mass screening of insect pools.

Original languageEnglish (US)
Pages (from-to)171-173
Number of pages3
JournalJournal of Tissue Culture Methods
Volume11
Issue number3
DOIs
StatePublished - Sep 1 1988

Keywords

  • bluetongue
  • C6/36
  • Culicoides
  • ELISA
  • immunofluorescence

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

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