Isolation of bluetongue virus from the Culicoides vector can provide valuable epidemiologic information. The methods described offer a practical procedure that ensures the preservation of the virus during field processing of the collection and an assay system that utilizes C6/36 cells, a clone derived from Aedes albopictus. The C6/36 cell line affords a sensitive isolation system and a practical virus detection system suitable for mass screening of insect pools.
ASJC Scopus subject areas
- Pathology and Forensic Medicine