TY - JOUR
T1 - Probing function in 3D neuronal cultures
T2 - A survey of 3D multielectrode array advances
AU - Lam, Doris
AU - Fischer, Nicholas O.
AU - Enright, Heather A.
N1 - Funding Information:
This work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344 through LDRD awards 17-SI-002, 20-ERD-009, and 21-LW-014.
Publisher Copyright:
© 2021 The Author(s)
PY - 2021/10
Y1 - 2021/10
N2 - Recent advances in microphysiological systems have made significant strides to include design features that reconstruct key elements found in the brain, and in parallel advance technologies to detect the activity of electrogenic cells that form neural networks. In particular, three-dimensional multielectrode arrays (3D MEAs) are being developed with increasing levels of spatial and temporal precision, difficult to achieve with current 2D MEAs, insertable MEA probes, and/or optical imaging of calcium dynamics. Thus, providing a means to monitor the flow of neural network activity within all three dimensions (X, Y, and Z) of the engineered tissue. In the last 6 years, 3D MEAs, using either bottom-up or top-down designs, have been developed to overcome the current technical challenges in monitoring the functionality of the in vitro systems. Herein, we will report on the design and application of novel 3D MEA prototypes for probing neural activity throughout the 3D neural tissue.
AB - Recent advances in microphysiological systems have made significant strides to include design features that reconstruct key elements found in the brain, and in parallel advance technologies to detect the activity of electrogenic cells that form neural networks. In particular, three-dimensional multielectrode arrays (3D MEAs) are being developed with increasing levels of spatial and temporal precision, difficult to achieve with current 2D MEAs, insertable MEA probes, and/or optical imaging of calcium dynamics. Thus, providing a means to monitor the flow of neural network activity within all three dimensions (X, Y, and Z) of the engineered tissue. In the last 6 years, 3D MEAs, using either bottom-up or top-down designs, have been developed to overcome the current technical challenges in monitoring the functionality of the in vitro systems. Herein, we will report on the design and application of novel 3D MEA prototypes for probing neural activity throughout the 3D neural tissue.
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U2 - 10.1016/j.coph.2021.08.003
DO - 10.1016/j.coph.2021.08.003
M3 - Review article
C2 - 34481335
AN - SCOPUS:85113924709
VL - 60
SP - 255
EP - 260
JO - Current Opinion in Pharmacology
JF - Current Opinion in Pharmacology
SN - 1471-4892
ER -