Probe-based fluorescence dosimetry of an antibody-dye conjugate to identify head and neck cancer as a first step to fluorescence-guided tissue preselection for pathological assessment

Naoki Nishio, Stan van Keulen, Nynke S. van den Berg, Guolan Lu, Ethan P. LaRochelle, Scott C. Davis, Brock A. Martin, Shayan Fakurnejad, Quan Zhou, Andrew C. Birkeland, Michael J. Kaplan, Vasu Divi, A. Dimitrios Colevas, Brian W. Pogue, Eben L. Rosenthal

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Background: Despite the rapid growth of fluorescence imaging, accurate sampling of tissue sections remains challenging. Development of novel technologies to improve intraoperative assessment of tissue is needed. Methods: A novel contact probe-based fluorescence dosimeter device, optimized for IRDye800CW quantification, was developed. After evaluation of the device in a phantom setup, its clinical value was defined ex vivo in patients with head and neck squamous cell carcinoma who received panitumumab-IRDye800CW. Results: Ten patients were enrolled with a total of 216 data points obtained. Final histopathology showed tumor in 119 spots and normal tissue in 97 spots. Fluorescence-to-excitation ratios in tumor tissue were more than three times higher than those in normal tissue. The area under the curve was 0.86 (95% CI: 0.81-0.91) for tumor detection. Conclusions: Fluorescence-guided tissue preselection using a fluorescence dosimeter could have substantial impact on tissue sampling for frozen section analysis and potentially reduce sampling errors.

Original languageEnglish (US)
JournalHead and Neck
DOIs
StateAccepted/In press - Jan 1 2019
Externally publishedYes

Keywords

  • EGFR
  • fluorescence dosimetry
  • fluorescence-guided tissue preselection
  • head and neck cancer
  • panitumumab

ASJC Scopus subject areas

  • Otorhinolaryngology

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    Nishio, N., van Keulen, S., van den Berg, N. S., Lu, G., LaRochelle, E. P., Davis, S. C., Martin, B. A., Fakurnejad, S., Zhou, Q., Birkeland, A. C., Kaplan, M. J., Divi, V., Colevas, A. D., Pogue, B. W., & Rosenthal, E. L. (Accepted/In press). Probe-based fluorescence dosimetry of an antibody-dye conjugate to identify head and neck cancer as a first step to fluorescence-guided tissue preselection for pathological assessment. Head and Neck. https://doi.org/10.1002/hed.25964