Pro-apoptotic and growth-inhibitory effect of IFN-β-overexpressing canine adipose tissue-derived mesenchymal stem cells against melanoma cells

Sei Myoung Han, Chong Woo Park, Jin Ok Ahn, Sang Chul Park, Woo Sung Jung, Kyoung Won Seo, Jeong Chan Ra, Sung Keun Kang, Hee Woo Lee, Hwa Young Youn

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Canine melanoma is the most common type of tumor in dogs. We investigated the effects of canine interferon-beta (cIFN-β)-overexpressing adipose tissuederived mesenchymal stem cells (cATMSCs) on apoptosis and proliferation of canine melanoma cells. Materials and Methods: Expression of IFN-β in cATMSCs was confirmed using reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assays. Flow cytometry was performed for cell-cycle analysis and apoptotic cell quantification of LMeC (melanoma) cells. Protein expression of cyclin D1, procaspase-3, activated caspase-3, and Bcl-2 homologous antagonist killer (Bak) was evaluated by western blot analysis. Results: Decreased proportions of cells in S- and G0/G1 phases were observed in parallel with decreased cyclin D1 expression in LMeC cells treated with cIFN-β-cATMSCconditioned media. Protein expression of active forms of caspase 3 and Bak increased in response to treatment with cIFN-β-cATMSC-conditioned media. Conclusion: IFN-β overexpression by cATMSCs was associated with proapoptotic and growth-inhibitory effects on canine melanoma cells. The antitumor effects of these cells have therapeutic potential for the treatment of canine melanoma.

Original languageEnglish (US)
Pages (from-to)4749-4756
Number of pages8
JournalAnticancer Research
Volume35
Issue number9
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

Mesenchymal Stromal Cells
Canidae
Adipose Tissue
Melanoma
Growth
Interferon-beta
Caspase 3
Cyclin D1
Cell Cycle Resting Phase
G1 Phase
Conditioned Culture Medium
Reverse Transcription
Cell Cycle
Flow Cytometry
Proteins
Therapeutics
Western Blotting
Enzyme-Linked Immunosorbent Assay
Dogs
Apoptosis

Keywords

  • Apoptosis
  • Cell cycle
  • Interferon β
  • Melanoma
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Pro-apoptotic and growth-inhibitory effect of IFN-β-overexpressing canine adipose tissue-derived mesenchymal stem cells against melanoma cells. / Han, Sei Myoung; Park, Chong Woo; Ahn, Jin Ok; Park, Sang Chul; Jung, Woo Sung; Seo, Kyoung Won; Ra, Jeong Chan; Kang, Sung Keun; Lee, Hee Woo; Youn, Hwa Young.

In: Anticancer Research, Vol. 35, No. 9, 01.01.2015, p. 4749-4756.

Research output: Contribution to journalArticle

Han, SM, Park, CW, Ahn, JO, Park, SC, Jung, WS, Seo, KW, Ra, JC, Kang, SK, Lee, HW & Youn, HY 2015, 'Pro-apoptotic and growth-inhibitory effect of IFN-β-overexpressing canine adipose tissue-derived mesenchymal stem cells against melanoma cells', Anticancer Research, vol. 35, no. 9, pp. 4749-4756.
Han, Sei Myoung ; Park, Chong Woo ; Ahn, Jin Ok ; Park, Sang Chul ; Jung, Woo Sung ; Seo, Kyoung Won ; Ra, Jeong Chan ; Kang, Sung Keun ; Lee, Hee Woo ; Youn, Hwa Young. / Pro-apoptotic and growth-inhibitory effect of IFN-β-overexpressing canine adipose tissue-derived mesenchymal stem cells against melanoma cells. In: Anticancer Research. 2015 ; Vol. 35, No. 9. pp. 4749-4756.
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abstract = "Background: Canine melanoma is the most common type of tumor in dogs. We investigated the effects of canine interferon-beta (cIFN-β)-overexpressing adipose tissuederived mesenchymal stem cells (cATMSCs) on apoptosis and proliferation of canine melanoma cells. Materials and Methods: Expression of IFN-β in cATMSCs was confirmed using reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assays. Flow cytometry was performed for cell-cycle analysis and apoptotic cell quantification of LMeC (melanoma) cells. Protein expression of cyclin D1, procaspase-3, activated caspase-3, and Bcl-2 homologous antagonist killer (Bak) was evaluated by western blot analysis. Results: Decreased proportions of cells in S- and G0/G1 phases were observed in parallel with decreased cyclin D1 expression in LMeC cells treated with cIFN-β-cATMSCconditioned media. Protein expression of active forms of caspase 3 and Bak increased in response to treatment with cIFN-β-cATMSC-conditioned media. Conclusion: IFN-β overexpression by cATMSCs was associated with proapoptotic and growth-inhibitory effects on canine melanoma cells. The antitumor effects of these cells have therapeutic potential for the treatment of canine melanoma.",
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AU - Park, Sang Chul

AU - Jung, Woo Sung

AU - Seo, Kyoung Won

AU - Ra, Jeong Chan

AU - Kang, Sung Keun

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AB - Background: Canine melanoma is the most common type of tumor in dogs. We investigated the effects of canine interferon-beta (cIFN-β)-overexpressing adipose tissuederived mesenchymal stem cells (cATMSCs) on apoptosis and proliferation of canine melanoma cells. Materials and Methods: Expression of IFN-β in cATMSCs was confirmed using reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assays. Flow cytometry was performed for cell-cycle analysis and apoptotic cell quantification of LMeC (melanoma) cells. Protein expression of cyclin D1, procaspase-3, activated caspase-3, and Bcl-2 homologous antagonist killer (Bak) was evaluated by western blot analysis. Results: Decreased proportions of cells in S- and G0/G1 phases were observed in parallel with decreased cyclin D1 expression in LMeC cells treated with cIFN-β-cATMSCconditioned media. Protein expression of active forms of caspase 3 and Bak increased in response to treatment with cIFN-β-cATMSC-conditioned media. Conclusion: IFN-β overexpression by cATMSCs was associated with proapoptotic and growth-inhibitory effects on canine melanoma cells. The antitumor effects of these cells have therapeutic potential for the treatment of canine melanoma.

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