Pro-apoptotic and growth-inhibitory effect of IFN-β-overexpressing canine adipose tissue-derived mesenchymal stem cells against melanoma cells

Sei Myoung Han, Chong Woo Park, Jin Ok Ahn, Sang Chul Park, Woo Sung Jung, Kyoung Won Seo, Jeong Chan Ra, Sung Keun Kang, Hee Woo Lee, Hwa Young Youn

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Background: Canine melanoma is the most common type of tumor in dogs. We investigated the effects of canine interferon-beta (cIFN-β)-overexpressing adipose tissuederived mesenchymal stem cells (cATMSCs) on apoptosis and proliferation of canine melanoma cells. Materials and Methods: Expression of IFN-β in cATMSCs was confirmed using reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assays. Flow cytometry was performed for cell-cycle analysis and apoptotic cell quantification of LMeC (melanoma) cells. Protein expression of cyclin D1, procaspase-3, activated caspase-3, and Bcl-2 homologous antagonist killer (Bak) was evaluated by western blot analysis. Results: Decreased proportions of cells in S- and G0/G1 phases were observed in parallel with decreased cyclin D1 expression in LMeC cells treated with cIFN-β-cATMSCconditioned media. Protein expression of active forms of caspase 3 and Bak increased in response to treatment with cIFN-β-cATMSC-conditioned media. Conclusion: IFN-β overexpression by cATMSCs was associated with proapoptotic and growth-inhibitory effects on canine melanoma cells. The antitumor effects of these cells have therapeutic potential for the treatment of canine melanoma.

Original languageEnglish (US)
Pages (from-to)4749-4756
Number of pages8
JournalAnticancer Research
Volume35
Issue number9
StatePublished - Jan 1 2015
Externally publishedYes

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Keywords

  • Apoptosis
  • Cell cycle
  • Interferon β
  • Melanoma
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Han, S. M., Park, C. W., Ahn, J. O., Park, S. C., Jung, W. S., Seo, K. W., Ra, J. C., Kang, S. K., Lee, H. W., & Youn, H. Y. (2015). Pro-apoptotic and growth-inhibitory effect of IFN-β-overexpressing canine adipose tissue-derived mesenchymal stem cells against melanoma cells. Anticancer Research, 35(9), 4749-4756.