Primate preimplantation embryo is a target for relaxin during early pregnancy

Catherine A. Vandevoort, Namdori R. Mtango, Keith E. Latham, Dennis R. Stewart

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: To determine whether preimplantation embryos are targets for relaxin secreted from the corpus luteum of the menstrual cycle. Design: Rhesus monkey oocytes obtained from females undergoing controlled ovarian hyperstimulation were inseminated, and the resulting embryos were cultured in medium with or without recombinant human relaxin (20 ng/mL) for 8 days. Setting: Research laboratory. Animal(s): Rhesus monkey. Intervention(s): Controlled ovarian stimulation to obtain oocytes for in vitro-produced embryos that were cultured with or without human recombinant relaxin. Main Outcome Measure(s): Rate of blastocyst development, percentage of blastocysts, and inner cell mass/trophectoderm cell ratio were measured on day 8 of culture. The presence of relaxin receptor (RXFP1) messenger RNA in eight-cell embryos was observed by array hybridization. Result(s): RXFP1 receptor expression was localized to the inner cell mass of blastocysts, as shown by immunohistochemistry. The percentage of embryos that developed to blastocyst and the inner cell mass/trophectoderm cell ratio was unchanged with relaxin supplementation; however, the relaxin-treated embryos developed into blastocysts significantly sooner than untreated embryos. Conclusion(s): These results are the first evidence that the preimplantation primate embryo is a target for relaxin and that the addition of relaxin to in vitro culture medium enhances rhesus monkey embryo development.

Original languageEnglish (US)
Pages (from-to)203-207
Number of pages5
JournalFertility and Sterility
Volume96
Issue number1
DOIs
StatePublished - Jul 2011

Fingerprint

Relaxin
Blastocyst
Primates
Embryonic Structures
Blastocyst Inner Cell Mass
Pregnancy
Macaca mulatta
Oocytes
Ovulation Induction
Corpus Luteum
Menstrual Cycle
Embryonic Development
Culture Media
Immunohistochemistry
Outcome Assessment (Health Care)
Messenger RNA
Research

Keywords

  • blastocyst
  • Gene expression
  • granulosa cells
  • rhesus macaque

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Primate preimplantation embryo is a target for relaxin during early pregnancy. / Vandevoort, Catherine A.; Mtango, Namdori R.; Latham, Keith E.; Stewart, Dennis R.

In: Fertility and Sterility, Vol. 96, No. 1, 07.2011, p. 203-207.

Research output: Contribution to journalArticle

Vandevoort, Catherine A. ; Mtango, Namdori R. ; Latham, Keith E. ; Stewart, Dennis R. / Primate preimplantation embryo is a target for relaxin during early pregnancy. In: Fertility and Sterility. 2011 ; Vol. 96, No. 1. pp. 203-207.
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