Abstract
Objective: To determine whether preimplantation embryos are targets for relaxin secreted from the corpus luteum of the menstrual cycle. Design: Rhesus monkey oocytes obtained from females undergoing controlled ovarian hyperstimulation were inseminated, and the resulting embryos were cultured in medium with or without recombinant human relaxin (20 ng/mL) for 8 days. Setting: Research laboratory. Animal(s): Rhesus monkey. Intervention(s): Controlled ovarian stimulation to obtain oocytes for in vitro-produced embryos that were cultured with or without human recombinant relaxin. Main Outcome Measure(s): Rate of blastocyst development, percentage of blastocysts, and inner cell mass/trophectoderm cell ratio were measured on day 8 of culture. The presence of relaxin receptor (RXFP1) messenger RNA in eight-cell embryos was observed by array hybridization. Result(s): RXFP1 receptor expression was localized to the inner cell mass of blastocysts, as shown by immunohistochemistry. The percentage of embryos that developed to blastocyst and the inner cell mass/trophectoderm cell ratio was unchanged with relaxin supplementation; however, the relaxin-treated embryos developed into blastocysts significantly sooner than untreated embryos. Conclusion(s): These results are the first evidence that the preimplantation primate embryo is a target for relaxin and that the addition of relaxin to in vitro culture medium enhances rhesus monkey embryo development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 203-207 |
| Number of pages | 5 |
| Journal | Fertility and Sterility |
| Volume | 96 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 2011 |
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Keywords
- blastocyst
- Gene expression
- granulosa cells
- rhesus macaque
ASJC Scopus subject areas
- Obstetrics and Gynecology
- Reproductive Medicine
Cite this
Primate preimplantation embryo is a target for relaxin during early pregnancy. / Vandevoort, Catherine A.; Mtango, Namdori R.; Latham, Keith E.; Stewart, Dennis R.
In: Fertility and Sterility, Vol. 96, No. 1, 07.2011, p. 203-207.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Primate preimplantation embryo is a target for relaxin during early pregnancy
AU - Vandevoort, Catherine A.
AU - Mtango, Namdori R.
AU - Latham, Keith E.
AU - Stewart, Dennis R.
PY - 2011/7
Y1 - 2011/7
N2 - Objective: To determine whether preimplantation embryos are targets for relaxin secreted from the corpus luteum of the menstrual cycle. Design: Rhesus monkey oocytes obtained from females undergoing controlled ovarian hyperstimulation were inseminated, and the resulting embryos were cultured in medium with or without recombinant human relaxin (20 ng/mL) for 8 days. Setting: Research laboratory. Animal(s): Rhesus monkey. Intervention(s): Controlled ovarian stimulation to obtain oocytes for in vitro-produced embryos that were cultured with or without human recombinant relaxin. Main Outcome Measure(s): Rate of blastocyst development, percentage of blastocysts, and inner cell mass/trophectoderm cell ratio were measured on day 8 of culture. The presence of relaxin receptor (RXFP1) messenger RNA in eight-cell embryos was observed by array hybridization. Result(s): RXFP1 receptor expression was localized to the inner cell mass of blastocysts, as shown by immunohistochemistry. The percentage of embryos that developed to blastocyst and the inner cell mass/trophectoderm cell ratio was unchanged with relaxin supplementation; however, the relaxin-treated embryos developed into blastocysts significantly sooner than untreated embryos. Conclusion(s): These results are the first evidence that the preimplantation primate embryo is a target for relaxin and that the addition of relaxin to in vitro culture medium enhances rhesus monkey embryo development.
AB - Objective: To determine whether preimplantation embryos are targets for relaxin secreted from the corpus luteum of the menstrual cycle. Design: Rhesus monkey oocytes obtained from females undergoing controlled ovarian hyperstimulation were inseminated, and the resulting embryos were cultured in medium with or without recombinant human relaxin (20 ng/mL) for 8 days. Setting: Research laboratory. Animal(s): Rhesus monkey. Intervention(s): Controlled ovarian stimulation to obtain oocytes for in vitro-produced embryos that were cultured with or without human recombinant relaxin. Main Outcome Measure(s): Rate of blastocyst development, percentage of blastocysts, and inner cell mass/trophectoderm cell ratio were measured on day 8 of culture. The presence of relaxin receptor (RXFP1) messenger RNA in eight-cell embryos was observed by array hybridization. Result(s): RXFP1 receptor expression was localized to the inner cell mass of blastocysts, as shown by immunohistochemistry. The percentage of embryos that developed to blastocyst and the inner cell mass/trophectoderm cell ratio was unchanged with relaxin supplementation; however, the relaxin-treated embryos developed into blastocysts significantly sooner than untreated embryos. Conclusion(s): These results are the first evidence that the preimplantation primate embryo is a target for relaxin and that the addition of relaxin to in vitro culture medium enhances rhesus monkey embryo development.
KW - blastocyst
KW - Gene expression
KW - granulosa cells
KW - rhesus macaque
UR - http://www.scopus.com/inward/record.url?scp=79959908102&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79959908102&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2011.05.016
DO - 10.1016/j.fertnstert.2011.05.016
M3 - Article
C2 - 21645893
AN - SCOPUS:79959908102
VL - 96
SP - 203
EP - 207
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 1
ER -