Abstract
The objective of this study was to identify cellular and organ targets of acute feline immunodeficiency virus (FIV) infection in vivo. Tissues of FIV- infected cats were studied at eight time points during the first 3 months after experimental infection. FIV nucleic acids were first detected by in situ hybridization 21 days after infection, approximately 1.5 weeks after lymph node enlargement was first observed and 3 weeks before the primary acute flu-like illness. The majority of FIV-infected cells were present in lymphoid organs, though low numbers of infected cells were noted in nonlymphoid organs as well. Germinal centers harbored many of the FIV- infected cells within lymphoid tissues. The thymic cortex was also a major site of early infection. Combined in situ hybridization and immunohistochemistry revealed that T lymphocytes were the primary target of early FIV infection in tissues of cats before the onset of clinical signs of acute illness. An unidentified population of mononuclear cells and a few macrophages were also infected. During the ensuing acute flu-like illness, the proportion of FIV-infected macrophages in tissues increased dramatically. This early shift in the predominant cellular localization of FIV from T lymphocytes to macrophages may be important for establishing viral persistence.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3080-3091 |
| Number of pages | 12 |
| Journal | Journal of Virology |
| Volume | 68 |
| Issue number | 5 |
| State | Published - 1994 |
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ASJC Scopus subject areas
- Immunology
Cite this
Primary stage of feline immunodeficiency virus infection : Viral dissemination and cellular targets. / Beebe, A. M.; Dua, N.; Faith, T. G.; Moore, Peter F; Pedersen, Niels C; Dandekar, Satya.
In: Journal of Virology, Vol. 68, No. 5, 1994, p. 3080-3091.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Primary stage of feline immunodeficiency virus infection
T2 - Viral dissemination and cellular targets
AU - Beebe, A. M.
AU - Dua, N.
AU - Faith, T. G.
AU - Moore, Peter F
AU - Pedersen, Niels C
AU - Dandekar, Satya
PY - 1994
Y1 - 1994
N2 - The objective of this study was to identify cellular and organ targets of acute feline immunodeficiency virus (FIV) infection in vivo. Tissues of FIV- infected cats were studied at eight time points during the first 3 months after experimental infection. FIV nucleic acids were first detected by in situ hybridization 21 days after infection, approximately 1.5 weeks after lymph node enlargement was first observed and 3 weeks before the primary acute flu-like illness. The majority of FIV-infected cells were present in lymphoid organs, though low numbers of infected cells were noted in nonlymphoid organs as well. Germinal centers harbored many of the FIV- infected cells within lymphoid tissues. The thymic cortex was also a major site of early infection. Combined in situ hybridization and immunohistochemistry revealed that T lymphocytes were the primary target of early FIV infection in tissues of cats before the onset of clinical signs of acute illness. An unidentified population of mononuclear cells and a few macrophages were also infected. During the ensuing acute flu-like illness, the proportion of FIV-infected macrophages in tissues increased dramatically. This early shift in the predominant cellular localization of FIV from T lymphocytes to macrophages may be important for establishing viral persistence.
AB - The objective of this study was to identify cellular and organ targets of acute feline immunodeficiency virus (FIV) infection in vivo. Tissues of FIV- infected cats were studied at eight time points during the first 3 months after experimental infection. FIV nucleic acids were first detected by in situ hybridization 21 days after infection, approximately 1.5 weeks after lymph node enlargement was first observed and 3 weeks before the primary acute flu-like illness. The majority of FIV-infected cells were present in lymphoid organs, though low numbers of infected cells were noted in nonlymphoid organs as well. Germinal centers harbored many of the FIV- infected cells within lymphoid tissues. The thymic cortex was also a major site of early infection. Combined in situ hybridization and immunohistochemistry revealed that T lymphocytes were the primary target of early FIV infection in tissues of cats before the onset of clinical signs of acute illness. An unidentified population of mononuclear cells and a few macrophages were also infected. During the ensuing acute flu-like illness, the proportion of FIV-infected macrophages in tissues increased dramatically. This early shift in the predominant cellular localization of FIV from T lymphocytes to macrophages may be important for establishing viral persistence.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028298084&partnerID=8YFLogxK
M3 - Article
C2 - 8151773
AN - SCOPUS:0028298084
VL - 68
SP - 3080
EP - 3091
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 5
ER -