Primary cultures of the dog's tracheal epithelium: Fine structure, fluid, and electrolyte transport

Jonathan Widdicombe, D. L. Coleman, W. E. Finkbeiner, D. S. Friend

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Cells from the dog's tracheal mucosa formed confluent epithelial sheets in culture. Typical tight junctions separated the apical from the basolateral portion of the cell membrane. The apical portion of the cell contained numerous short microvilli and a pronounced glycocalyx. The basolateral portion of the plasma membrane was unspecialized except for extensive gap junctions between cells. Freeze-fracture showed that the cultured cells lacked the basolateral square (orthogonal) arrays of the original tissue, particles previously implicated in ion transport. Formation of domes indicated the presence of active fluid absorption. Domes appeared between days 4 and 8 of culture and persisted for about 1 week. Cell sheets showed a transepithelial resistance of ≈ 400 Ω·cm2 and a short-circuit current (Isc) of ≈ 5 μA·cm-2. The effects of transport inhibitors indicated that both active Na absorption and active Cl secretion were present. Isc was increased by isoproterenol, prostaglandins E2 and F, vasoactive intestinal peptide, dibutyryl cyclic AMP, leukotrienes C4 and D4, and bradykinin. These changes were probably due to stimulation of active Cl secretion.

Original languageEnglish (US)
Pages (from-to)95-103
Number of pages9
JournalCell and Tissue Research
Volume247
Issue number1
DOIs
StatePublished - Jan 1987
Externally publishedYes

Keywords

  • Airway epithelium
  • Cell culture
  • Chloride secretion
  • Dog
  • Fluid absorption
  • Square arrays

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

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