Primary culture of human retinal glia.

A. E. Aotaki-Keen, A. K. Harvey, E. de Juan, Leonard M Hjelmeland

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Glial cells of the human retina participate in various pathologic processes characterized by cell migration, proliferation, and extracellular matrix production. To study these events in vitro, a procedure was developed to obtain primary cultures of human retinal glial cells. The cultures resulting from the processing of 130 globes contained cells with variable morphology including bipolar and multipolar or stellate cells. Most cells in the primary culture were labeled with antisera to the glial fibrillary acidic protein. The cultures were also examined with antibodies directed against factor VIII-related antigen and muscle-specific actin to determine the presence of endothelial cells and pericytes. A variable contamination of cells staining for the latter was found in these cultures (usually less than 10%). Together, these data indicated that the primary cultures arose principally from glial cells of the human retina but did not precisely identify the cell of origin.

Original languageEnglish (US)
Pages (from-to)1733-1738
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume32
Issue number6
StatePublished - May 1991

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Neuroglia
Retina
Pericytes
Primary Cell Culture
Glial Fibrillary Acidic Protein
von Willebrand Factor
Pathologic Processes
Cell Movement
Extracellular Matrix
Actins
Immune Sera
Endothelial Cells
Cell Proliferation
Staining and Labeling
Muscles
Antibodies

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Aotaki-Keen, A. E., Harvey, A. K., de Juan, E., & Hjelmeland, L. M. (1991). Primary culture of human retinal glia. Investigative Ophthalmology and Visual Science, 32(6), 1733-1738.

Primary culture of human retinal glia. / Aotaki-Keen, A. E.; Harvey, A. K.; de Juan, E.; Hjelmeland, Leonard M.

In: Investigative Ophthalmology and Visual Science, Vol. 32, No. 6, 05.1991, p. 1733-1738.

Research output: Contribution to journalArticle

Aotaki-Keen, AE, Harvey, AK, de Juan, E & Hjelmeland, LM 1991, 'Primary culture of human retinal glia.', Investigative Ophthalmology and Visual Science, vol. 32, no. 6, pp. 1733-1738.
Aotaki-Keen AE, Harvey AK, de Juan E, Hjelmeland LM. Primary culture of human retinal glia. Investigative Ophthalmology and Visual Science. 1991 May;32(6):1733-1738.
Aotaki-Keen, A. E. ; Harvey, A. K. ; de Juan, E. ; Hjelmeland, Leonard M. / Primary culture of human retinal glia. In: Investigative Ophthalmology and Visual Science. 1991 ; Vol. 32, No. 6. pp. 1733-1738.
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