Abstract
Site-specific responses of bronchoalveolar and peripheral blood lymphocyte subsets were compared during primary and anamnestic immune responses against live Pasteurella haemolytica A1 (Ph1). Eight 1-year old calves were sequentially exposed intrabronchially with aerosolized Ph1 on days 0, 14, and 21, and two calves were sham exposed. Bronchoalveolar and peripheral blood lymphocytes were analyzed before each Phi exposure, and on days 3 and 7 post exposure using single and two-color flow cytometry to identify CD2, CD4+, CD8+, CD21+, CD45R+, CD25+ and γδ lymphocyte subsets. Significant differences (p < 0.05) in bronchoalveolar and peripheral blood lymphocyte subsets were observed before Ph1 exposure. Subsequent aerosol exposures, resulted in significant (p <0.05) changes in bronchoalveolar lymphocyte subsets and the CD4:CD8 bronchoalveolar lymphocyte ratio, but concomitant changes were not observed in peripheral blood lymphocytes. Expression of CD2, CD4 and CD8 lymphocyte differentiation antigens was consistently lower and more heterogeneous on bronchoalveolar lymphocytes. Differential analysis of bronchoalveolar leukocytes revealed a significant increase in bronchoalveolar lymphocytes and neutrophils during anamnestic responses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 161-170 |
| Number of pages | 10 |
| Journal | Veterinary Immunology and Immunopathology |
| Volume | 67 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 1999 |
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Keywords
- Bovine
- Bronchoalveolar
- Lymphocyte
- Pasteurella
- Phenotype
- Respiratory disease
ASJC Scopus subject areas
- Animal Science and Zoology
- Immunology
- veterinary(all)
Cite this
Primary and anamnestic responses of bovine bronchoalveolar and peripheral blood lymphocyte subsets to aerosolized Pasteurella haemolytica A1. / McBride, Jere W.; Corstvet, Richard E.; Taylor, Bernadette C.; Osburn, Bennie.
In: Veterinary Immunology and Immunopathology, Vol. 67, No. 2, 01.02.1999, p. 161-170.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Primary and anamnestic responses of bovine bronchoalveolar and peripheral blood lymphocyte subsets to aerosolized Pasteurella haemolytica A1
AU - McBride, Jere W.
AU - Corstvet, Richard E.
AU - Taylor, Bernadette C.
AU - Osburn, Bennie
PY - 1999/2/1
Y1 - 1999/2/1
N2 - Site-specific responses of bronchoalveolar and peripheral blood lymphocyte subsets were compared during primary and anamnestic immune responses against live Pasteurella haemolytica A1 (Ph1). Eight 1-year old calves were sequentially exposed intrabronchially with aerosolized Ph1 on days 0, 14, and 21, and two calves were sham exposed. Bronchoalveolar and peripheral blood lymphocytes were analyzed before each Phi exposure, and on days 3 and 7 post exposure using single and two-color flow cytometry to identify CD2, CD4+, CD8+, CD21+, CD45R+, CD25+ and γδ lymphocyte subsets. Significant differences (p < 0.05) in bronchoalveolar and peripheral blood lymphocyte subsets were observed before Ph1 exposure. Subsequent aerosol exposures, resulted in significant (p <0.05) changes in bronchoalveolar lymphocyte subsets and the CD4:CD8 bronchoalveolar lymphocyte ratio, but concomitant changes were not observed in peripheral blood lymphocytes. Expression of CD2, CD4 and CD8 lymphocyte differentiation antigens was consistently lower and more heterogeneous on bronchoalveolar lymphocytes. Differential analysis of bronchoalveolar leukocytes revealed a significant increase in bronchoalveolar lymphocytes and neutrophils during anamnestic responses.
AB - Site-specific responses of bronchoalveolar and peripheral blood lymphocyte subsets were compared during primary and anamnestic immune responses against live Pasteurella haemolytica A1 (Ph1). Eight 1-year old calves were sequentially exposed intrabronchially with aerosolized Ph1 on days 0, 14, and 21, and two calves were sham exposed. Bronchoalveolar and peripheral blood lymphocytes were analyzed before each Phi exposure, and on days 3 and 7 post exposure using single and two-color flow cytometry to identify CD2, CD4+, CD8+, CD21+, CD45R+, CD25+ and γδ lymphocyte subsets. Significant differences (p < 0.05) in bronchoalveolar and peripheral blood lymphocyte subsets were observed before Ph1 exposure. Subsequent aerosol exposures, resulted in significant (p <0.05) changes in bronchoalveolar lymphocyte subsets and the CD4:CD8 bronchoalveolar lymphocyte ratio, but concomitant changes were not observed in peripheral blood lymphocytes. Expression of CD2, CD4 and CD8 lymphocyte differentiation antigens was consistently lower and more heterogeneous on bronchoalveolar lymphocytes. Differential analysis of bronchoalveolar leukocytes revealed a significant increase in bronchoalveolar lymphocytes and neutrophils during anamnestic responses.
KW - Bovine
KW - Bronchoalveolar
KW - Lymphocyte
KW - Pasteurella
KW - Phenotype
KW - Respiratory disease
UR - http://www.scopus.com/inward/record.url?scp=0033084121&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033084121&partnerID=8YFLogxK
U2 - 10.1016/S0165-2427(98)00218-9
DO - 10.1016/S0165-2427(98)00218-9
M3 - Article
C2 - 10077422
AN - SCOPUS:0033084121
VL - 67
SP - 161
EP - 170
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
SN - 0165-2427
IS - 2
ER -