Primary afferent nerves mediate in part β-adrenoceptor stimulation-induced mesenteric hyperemia in rats

Kyoji Seno, Kan Lam, Joseph Leung, Felix W. Leung

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We tested the hypothesis that in the rat duodenum and intestine the mesenteric hyperemia due to β-adrenoceptor stimulation is mediated by capsaicin-sensitive afferent nerves. Superior mesenteric artery blood flow was measured by pulsed Doppler flowmetry in the anesthetized rat. Functional ablation of afferent nerves was accomplished by subcutaneous 125 mg/kg capsaicin pretreatment 10 to 14 days before blood flow studies. Blockade of capsaicin-sensitive cation-selective ion channels of the duodenal and intestinal mucosal afferent nerves was achieved by intraduodenal 0.1% ruthenium red given 15 min prior to the intraduodenal administration of 5 mg/kg isoproterenol. Functional ablation of the afferent nerves and blockade of the capsaicin-sensitive cation-selective ion channels alone or in combination resulted in a significant reduction of mesenteric hyperemia induced by intraduodenal isoproterenol. These data support the hypothesis that β-adrenoceptor stimulation by intraduodenal isoproterenol induces mesenteric hyperemia in part through afferent nerves in the rat duodenal and intestinal mucosa. The results suggest for the first time a link between β-adrenoceptor function and peripheral capsaicin-sensitive afferent nerve-mediated mechanism in the rat gut.

Original languageEnglish (US)
Pages (from-to)1203-1209
Number of pages7
JournalLife Sciences
Volume59
Issue number15
DOIs
StatePublished - Sep 6 1996

Fingerprint

Capsaicin
Hyperemia
Adrenergic Receptors
Rats
Isoproterenol
Ablation
Ion Channels
Cations
Blood
Ruthenium Red
Superior Mesenteric Artery
Rheology
Nerve Block
Intestinal Mucosa
Duodenum
Intestines

Keywords

  • capsaicin
  • duodenum
  • intestine
  • isoproterenol
  • ruthenium red

ASJC Scopus subject areas

  • Pharmacology

Cite this

Primary afferent nerves mediate in part β-adrenoceptor stimulation-induced mesenteric hyperemia in rats. / Seno, Kyoji; Lam, Kan; Leung, Joseph; Leung, Felix W.

In: Life Sciences, Vol. 59, No. 15, 06.09.1996, p. 1203-1209.

Research output: Contribution to journalArticle

Seno, Kyoji ; Lam, Kan ; Leung, Joseph ; Leung, Felix W. / Primary afferent nerves mediate in part β-adrenoceptor stimulation-induced mesenteric hyperemia in rats. In: Life Sciences. 1996 ; Vol. 59, No. 15. pp. 1203-1209.
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