Prevention of siderophore- mediated gut-derived sepsis due to P. aeruginosa can be achieved without iron provision by maintaining local phosphate abundance: Role of pH

Kathleen Romanowski, Alexander Zaborin, Hoylan Fernandez, Valeriy Poroyko, Vesta Valuckaite, Svetlana Gerdes, Donald C. Liu, Olga Y. Zaborina, John C. Alverdy

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: During extreme physiological stress, the intestinal tract can be transformed into a harsh environment characterized by regio- spatial alterations in oxygen, pH, and phosphate concentration. When the human intestine is exposed to extreme medical interventions, the normal flora becomes replaced by pathogenic species whose virulence can be triggered by various physico-chemical cues leading to lethal sepsis. We previously demonstrated that phosphate depletion develops in the mouse intestine following surgical injury and triggers intestinal P. aeruginosa to express a lethal phenotype that can be prevented by oral phosphate ([Pi]) supplementation. Results: In this study we examined the role of pH in the protective effect of [Pi] supplementation as it has been shown to be increased in the distal gut following surgical injury. Surgically injured mice drinking 25 mM [Pi] at pH 7.5 and intestinally inoculated with P. aeruginosa had increased mortality compared to mice drinking 25 mM [Pi] at pH 6.0 (p < 0.05). This finding was confirmed in C. elegans. Transcriptional analysis of P. aeruginosa demonstrated enhanced expression of various genes involved in media alkalization at pH 6.0 and a global increase in the expression of all iron-related genes at pH 7.5. Maintaining the pH at 6.0 via phosphate supplementation led to significant attenuation of iron-related genes as demonstrated by microarray and confirmed by QRT-PCR analyses. Conclusion: Taken together, these data demonstrate that increase in pH in distal intestine of physiologically stressed host colonized by P. aeruginosa can lead to the expression of siderophore-related virulence in bacteria that can be prevented without providing iron by maintaining local phosphate abundance at pH 6.0. This finding is particularly important as provision of exogenous iron has been shown to have untoward effects when administered to critically ill and septic patients. Given that phosphate, pH, and iron are near universal cues that dictate the virulence status of a broad range of microorganisms relevant to serious gut origin infection and sepsis in critically ill patients, the maintenance of phosphate and pH at appropriate physiologic levels to prevent virulence activation in a site specific manner can be considered as a novel anti-infective therapy in at risk patients.

Original languageEnglish (US)
Article number212
JournalBMC Microbiology
Volume11
DOIs
StatePublished - Oct 4 2011
Externally publishedYes

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Siderophores
Sepsis
Iron
Phosphates
Virulence
Intestines
Intraoperative Complications
Critical Illness
Drinking
Cues
Physiological Stress
Genes
Maintenance

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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Prevention of siderophore- mediated gut-derived sepsis due to P. aeruginosa can be achieved without iron provision by maintaining local phosphate abundance : Role of pH. / Romanowski, Kathleen; Zaborin, Alexander; Fernandez, Hoylan; Poroyko, Valeriy; Valuckaite, Vesta; Gerdes, Svetlana; Liu, Donald C.; Zaborina, Olga Y.; Alverdy, John C.

In: BMC Microbiology, Vol. 11, 212, 04.10.2011.

Research output: Contribution to journalArticle

Romanowski, Kathleen ; Zaborin, Alexander ; Fernandez, Hoylan ; Poroyko, Valeriy ; Valuckaite, Vesta ; Gerdes, Svetlana ; Liu, Donald C. ; Zaborina, Olga Y. ; Alverdy, John C. / Prevention of siderophore- mediated gut-derived sepsis due to P. aeruginosa can be achieved without iron provision by maintaining local phosphate abundance : Role of pH. In: BMC Microbiology. 2011 ; Vol. 11.
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AU - Poroyko, Valeriy

AU - Valuckaite, Vesta

AU - Gerdes, Svetlana

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AU - Alverdy, John C.

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