Prevention of premature ovarian failure and osteoporosis induced by irradiation using allogeneic ovarian/bone marrow transplantation

Wei Feng, Yunze Cui, Hongsheng Zhan, Ming Shi, Wenhao Cui, Kequan Guo, Qing Li, Changye Song, Yuming Zhang, Takahide Mori, M. Eric Gershwin, Nader G. Abraham, Susumu Ikehara

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Two side effects of irradiation are premature ovarian failure (POF) and osteoporosis, both of which are concerns not only clinically, for patients, but also experimentally, for animals. We examine whether bone marrow transplantation (BMT) can correct the POF induced by radiation and also address whether allogeneic ovarian transplantation (OT) can modulate the adverse effects of radiotherapy. Methods: Eight-week-old female C57BL/6 mice were lethally irradiated with 6 Gy ×2, and then injected with allogeneic bone marrow cells into their bone marrow cavity using our previously described intrabone marrow (IBM)-BMT technique. Allogeneic ovaries were simultaneously transplanted under the renal capsules of the mice. Results: Three months after the transplantation, we noted that hematopoietic and lymphoid cells had been successfully reconstituted. The ovaries transplanted under the renal capsules demonstrated signs of development with a large number of differentiating follicles at different stages of development. Importantly, the total bone mineral density of the tibia in the "IBM-BMT+OT" (BMT/OT) group remained normal. However, the reproductive function of the recipient mice was not restored, despite the presence of many immature oocytes in the host ovaries in the BMT/OT group. In the BMT group, no oocytes were found in the host ovaries. ConclusionS: These findings suggest that IBM-BMT with ovarian allografts can be advantageous for young women with POF and osteopenia or osteoporosis that is due to chemotherapy and radiotherapy for malignant diseases.

Original languageEnglish (US)
Pages (from-to)395-401
Number of pages7
JournalTransplantation
Volume89
Issue number4
DOIs
StatePublished - Feb 2010

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Primary Ovarian Insufficiency
Bone Marrow Transplantation
Osteoporosis
Ovary
Transplantation
Bone Marrow
Oocytes
Capsules
Radiotherapy
Kidney
Metabolic Bone Diseases
Homologous Transplantation
Tibia
Inbred C57BL Mouse
Bone Marrow Cells
Bone Density
Allografts
Lymphocytes
Radiation
Drug Therapy

Keywords

  • Allogeneic ovarian transplantation
  • Intra-bone marrow-bone marrow transplantation
  • Oocyte renewal
  • Osteoporosis
  • Premature ovarian failure

ASJC Scopus subject areas

  • Transplantation

Cite this

Prevention of premature ovarian failure and osteoporosis induced by irradiation using allogeneic ovarian/bone marrow transplantation. / Feng, Wei; Cui, Yunze; Zhan, Hongsheng; Shi, Ming; Cui, Wenhao; Guo, Kequan; Li, Qing; Song, Changye; Zhang, Yuming; Mori, Takahide; Gershwin, M. Eric; Abraham, Nader G.; Ikehara, Susumu.

In: Transplantation, Vol. 89, No. 4, 02.2010, p. 395-401.

Research output: Contribution to journalArticle

Feng, W, Cui, Y, Zhan, H, Shi, M, Cui, W, Guo, K, Li, Q, Song, C, Zhang, Y, Mori, T, Gershwin, ME, Abraham, NG & Ikehara, S 2010, 'Prevention of premature ovarian failure and osteoporosis induced by irradiation using allogeneic ovarian/bone marrow transplantation', Transplantation, vol. 89, no. 4, pp. 395-401. https://doi.org/10.1097/TP.0b013e3181ca86bb
Feng, Wei ; Cui, Yunze ; Zhan, Hongsheng ; Shi, Ming ; Cui, Wenhao ; Guo, Kequan ; Li, Qing ; Song, Changye ; Zhang, Yuming ; Mori, Takahide ; Gershwin, M. Eric ; Abraham, Nader G. ; Ikehara, Susumu. / Prevention of premature ovarian failure and osteoporosis induced by irradiation using allogeneic ovarian/bone marrow transplantation. In: Transplantation. 2010 ; Vol. 89, No. 4. pp. 395-401.
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AU - Guo, Kequan

AU - Li, Qing

AU - Song, Changye

AU - Zhang, Yuming

AU - Mori, Takahide

AU - Gershwin, M. Eric

AU - Abraham, Nader G.

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AB - Background: Two side effects of irradiation are premature ovarian failure (POF) and osteoporosis, both of which are concerns not only clinically, for patients, but also experimentally, for animals. We examine whether bone marrow transplantation (BMT) can correct the POF induced by radiation and also address whether allogeneic ovarian transplantation (OT) can modulate the adverse effects of radiotherapy. Methods: Eight-week-old female C57BL/6 mice were lethally irradiated with 6 Gy ×2, and then injected with allogeneic bone marrow cells into their bone marrow cavity using our previously described intrabone marrow (IBM)-BMT technique. Allogeneic ovaries were simultaneously transplanted under the renal capsules of the mice. Results: Three months after the transplantation, we noted that hematopoietic and lymphoid cells had been successfully reconstituted. The ovaries transplanted under the renal capsules demonstrated signs of development with a large number of differentiating follicles at different stages of development. Importantly, the total bone mineral density of the tibia in the "IBM-BMT+OT" (BMT/OT) group remained normal. However, the reproductive function of the recipient mice was not restored, despite the presence of many immature oocytes in the host ovaries in the BMT/OT group. In the BMT group, no oocytes were found in the host ovaries. ConclusionS: These findings suggest that IBM-BMT with ovarian allografts can be advantageous for young women with POF and osteopenia or osteoporosis that is due to chemotherapy and radiotherapy for malignant diseases.

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