Prevention of cold ischemia/rewarming-induced ERK 1/2, p38 kinase and HO-1 activation by trophic factor supplementation of UW solution

Young Sam Kwon, John D. Foley, Paul Russell, Jonathan F. McAnulty, Christopher J Murphy

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8 Scopus citations

Abstract

We have previously shown that trophic factor supplementation (TFS) of University of Wisconsin (UW) solution reduced early apoptotic changes in vascular endothelial cells. Here, we examine the effect of TFS on cell signaling pathways related to cell growth, differentiation, and apoptosis after cold ischemic storage. In this study, the effect of TFS on the phosphorylation of signaling molecules ERK (extracellular regulated-signaling kinase) 1/2 and p38 MAPK (mitogen activated protein kinases) and of HO-1 (hemeoxygenase-1), relative to changes seen in unmodified UW solution, were determined by Western blot in cells stored under cold ischemic conditions. Primary cultures of canine kidney proximal tubule cells (CKPTC) and human umbilical vein endothelial cells (HUVEC) were used in this study. There was a significant decrease, relative to UW solution, after 1 min rewarming in ERK 1 and 2 activity in CKPTCs. For p38 MAPK, a significant decrease after 5 min rewarming was seen in CKPTC (p < 0.05) while significant reductions relative to UW solution were seen in HUVECs after both 1 and 5 min rewarming (p < 0.05). Phosphorylated HO-1 was also decreased by 43% and 50% in HUVECs, relative to UW solution, after 1 and 5 min rewarming (p < 0.05 at each time point). Collectively, TFS not only limits ERK 1/2 and p38 MAPK activity induced by cold ischemic injury and subsequent rewarming, but also substantially restricted increases in HO-1 phosphorylation.

Original languageEnglish (US)
Pages (from-to)72-74
Number of pages3
JournalCryobiology
Volume57
Issue number1
DOIs
StatePublished - Aug 2008
Externally publishedYes

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Keywords

  • Cold ischemia
  • Endothelial cells
  • ERK 1/2
  • Growth factors
  • Hypothermia
  • Kidney cells
  • MAPK
  • p38 MAPK
  • Trophic factors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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