Prevalent, Dynamic, and Conserved R-Loop Structures Associate with Specific Epigenomic Signatures in Mammals

Lionel A. Sanz, Stella R. Hartono, Yoong Wearn Lim, Sandra Steyaert, Aparna Rajpurkar, Paul A. Ginno, Xiaoqin Xu, Frederic Chedin

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

R-loops are three-stranded nucleic acid structures formed upon annealing of an RNA strand to one strand of duplex DNA. We profiled R-loops using a high-resolution, strand-specific methodology in human and mouse cell types. R-loops are prevalent, collectively occupying up to 5% of mammalian genomes. R-loop formation occurs over conserved genic hotspots such as promoter and terminator regions of poly(A)-dependent genes. In most cases, R-loops occur co-transcriptionally and undergo dynamic turnover. Detailed epigenomic profiling revealed that R-loops associate with specific chromatin signatures. At promoters, R-loops associate with a hyper-accessible state characteristic of unmethylated CpG island promoters. By contrast, terminal R-loops associate with an enhancer- and insulator-like state and define a broad class of transcription terminators. Together, this suggests that the retention of nascent RNA transcripts at their site of expression represents an abundant, dynamic, and programmed component of the mammalian chromatin that affects chromatin patterning and the control of gene expression.

Original languageEnglish (US)
Pages (from-to)167-178
Number of pages12
JournalMolecular Cell
Volume63
Issue number1
DOIs
StatePublished - Jul 7 2016

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Epigenomics
Chromatin
Mammals
Genetic Terminator Regions
RNA
CpG Islands
Poly A
Genetic Promoter Regions
Nucleic Acids
Genome
Gene Expression
DNA
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Prevalent, Dynamic, and Conserved R-Loop Structures Associate with Specific Epigenomic Signatures in Mammals. / Sanz, Lionel A.; Hartono, Stella R.; Lim, Yoong Wearn; Steyaert, Sandra; Rajpurkar, Aparna; Ginno, Paul A.; Xu, Xiaoqin; Chedin, Frederic.

In: Molecular Cell, Vol. 63, No. 1, 07.07.2016, p. 167-178.

Research output: Contribution to journalArticle

Sanz, Lionel A. ; Hartono, Stella R. ; Lim, Yoong Wearn ; Steyaert, Sandra ; Rajpurkar, Aparna ; Ginno, Paul A. ; Xu, Xiaoqin ; Chedin, Frederic. / Prevalent, Dynamic, and Conserved R-Loop Structures Associate with Specific Epigenomic Signatures in Mammals. In: Molecular Cell. 2016 ; Vol. 63, No. 1. pp. 167-178.
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