Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California

Jarasvech Chinsangaram, Caroline E. Schore, Walter Guterbock, Leon D. Weaver, Bennie Osburn

Research output: Contribution to journal › Article

12 Citations (Scopus)

Abstract

136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.

Original languageEnglish (US)
Pages (from-to)93-103
Number of pages11
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume18
Issue number2
DOIs
StatePublished - Jan 1 1995

Fingerprint

Rotavirus B
dairy calves
Rotavirus
Feces
feces
calves
Polyacrylamide Gel Electrophoresis
polyacrylamide gel electrophoresis
sampling
Reverse Transcription
ranching
dairy herds
Polymerase Chain Reaction
reverse transcriptase polymerase chain reaction

Keywords

  • Group A bovine rotavirus
  • group B bovine rotavirus
  • polyacrylamide gel electrophoresis (PAGE)
  • reverse transcription-polymerase chain reaction (RT-PCR)
  • rotaviral diarrhea
  • short electropherotype

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • veterinary(all)
  • Infectious Diseases

Cite this

Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California. / Chinsangaram, Jarasvech; Schore, Caroline E.; Guterbock, Walter; Weaver, Leon D.; Osburn, Bennie.

In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 18, No. 2, 01.01.1995, p. 93-103.

Research output: Contribution to journal › Article

Chinsangaram, J, Schore, CE, Guterbock, W, Weaver, LD & Osburn, B 1995, 'Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California', Comparative Immunology, Microbiology and Infectious Diseases, vol. 18, no. 2, pp. 93-103. https://doi.org/10.1016/0147-9571(95)98850-H
Chinsangaram J, Schore CE, Guterbock W, Weaver LD, Osburn B. Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California. Comparative Immunology, Microbiology and Infectious Diseases. 1995 Jan 1;18(2):93-103. https://doi.org/10.1016/0147-9571(95)98850-H
Chinsangaram, Jarasvech ; Schore, Caroline E. ; Guterbock, Walter ; Weaver, Leon D. ; Osburn, Bennie. / Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California. In: Comparative Immunology, Microbiology and Infectious Diseases. 1995 ; Vol. 18, No. 2. pp. 93-103.
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