Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California

Jarasvech Chinsangaram; Caroline E. Schore; Walter Guterbock; Leon D. Weaver; Bennie Osburn

doi:10.1016/0147-9571(95)98850-H

Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California

Jarasvech Chinsangaram, Caroline E. Schore, Walter Guterbock, Leon D. Weaver, Bennie Osburn

UC Davis School of Medicine

Research output: Contribution to journal › Article

12 Scopus citations

Abstract

136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.

Original language	English (US)
Pages (from-to)	93-103
Number of pages	11
Journal	Comparative Immunology, Microbiology and Infectious Diseases
Volume	18
Issue number	2
DOIs	https://doi.org/10.1016/0147-9571(95)98850-H
State	Published - Jan 1 1995

Keywords

Group A bovine rotavirus
group B bovine rotavirus
polyacrylamide gel electrophoresis (PAGE)
reverse transcription-polymerase chain reaction (RT-PCR)
rotaviral diarrhea
short electropherotype

ASJC Scopus subject areas

Immunology and Allergy
Microbiology
Immunology
veterinary(all)
Infectious Diseases

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Chinsangaram, J., Schore, C. E., Guterbock, W., Weaver, L. D., & Osburn, B. (1995). Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California. Comparative Immunology, Microbiology and Infectious Diseases, 18(2), 93-103. https://doi.org/10.1016/0147-9571(95)98850-H

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abstract = "136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.",

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AU - Chinsangaram, Jarasvech

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AU - Guterbock, Walter

AU - Weaver, Leon D.

AU - Osburn, Bennie

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N2 - 136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.

AB - 136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.

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