Abstract
136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 93-103 |
| Number of pages | 11 |
| Journal | Comparative Immunology, Microbiology and Infectious Diseases |
| Volume | 18 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1995 |
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Keywords
- Group A bovine rotavirus
- group B bovine rotavirus
- polyacrylamide gel electrophoresis (PAGE)
- reverse transcription-polymerase chain reaction (RT-PCR)
- rotaviral diarrhea
- short electropherotype
ASJC Scopus subject areas
- Immunology and Allergy
- Microbiology
- Immunology
- veterinary(all)
- Infectious Diseases