Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California

Jarasvech Chinsangaram, Caroline E. Schore, Walter Guterbock, Leon D. Weaver, Bennie Osburn

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.

Original languageEnglish (US)
Pages (from-to)93-103
Number of pages11
JournalComparative Immunology, Microbiology and Infectious Diseases
Issue number2
StatePublished - Jan 1 1995


  • Group A bovine rotavirus
  • group B bovine rotavirus
  • polyacrylamide gel electrophoresis (PAGE)
  • reverse transcription-polymerase chain reaction (RT-PCR)
  • rotaviral diarrhea
  • short electropherotype

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • veterinary(all)
  • Infectious Diseases


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