Abstract
136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 93-103 |
| Number of pages | 11 |
| Journal | Comparative Immunology, Microbiology and Infectious Diseases |
| Volume | 18 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1995 |
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Keywords
- Group A bovine rotavirus
- group B bovine rotavirus
- polyacrylamide gel electrophoresis (PAGE)
- reverse transcription-polymerase chain reaction (RT-PCR)
- rotaviral diarrhea
- short electropherotype
ASJC Scopus subject areas
- Immunology and Allergy
- Microbiology
- Immunology
- veterinary(all)
- Infectious Diseases
Cite this
Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California. / Chinsangaram, Jarasvech; Schore, Caroline E.; Guterbock, Walter; Weaver, Leon D.; Osburn, Bennie.
In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 18, No. 2, 01.01.1995, p. 93-103.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California
AU - Chinsangaram, Jarasvech
AU - Schore, Caroline E.
AU - Guterbock, Walter
AU - Weaver, Leon D.
AU - Osburn, Bennie
PY - 1995/1/1
Y1 - 1995/1/1
N2 - 136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.
AB - 136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.
KW - Group A bovine rotavirus
KW - group B bovine rotavirus
KW - polyacrylamide gel electrophoresis (PAGE)
KW - reverse transcription-polymerase chain reaction (RT-PCR)
KW - rotaviral diarrhea
KW - short electropherotype
UR - http://www.scopus.com/inward/record.url?scp=0028938861&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028938861&partnerID=8YFLogxK
U2 - 10.1016/0147-9571(95)98850-H
DO - 10.1016/0147-9571(95)98850-H
M3 - Article
C2 - 7621673
AN - SCOPUS:0028938861
VL - 18
SP - 93
EP - 103
JO - Comparative Immunology, Microbiology and Infectious Diseases
JF - Comparative Immunology, Microbiology and Infectious Diseases
SN - 0147-9571
IS - 2
ER -