Prevalence of Enterotropic and Polytropic Mouse Hepatitis Virus in Enzootically Infected Mouse Colonies

Felix R. Homberger, Linong Zhang, Stephen W Barthold

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Mouse hepatitis virus (MHV) causes the most prevalent viral infection in contemporary laboratory mouse colonies. According to their primary replication site, different strains of MHV segregate into two overlapping biotypes, enterotropic and polytropic. These two groups vary greatly in disease pattern, pathogenicity, immune response, and duration of infection. Historically, the polytropic MHV strains represent the extensively studied prototype strains that have minimal enterotropism, whereas enterotropic MHV strains have been less characterized. Anecdotal reports suggest that most MHV strains encountered today belong to the enterotropic biotype. We have identified 15 isolates of MHV from 19 independent enzootically infected mouse colonies. Sequencing of a variable region of the nucleoprotein (N) gene of each isolate confirmed that all were independent genetic variants. The principal tissue tropism of the new isolates was determined by experimental inoculation of infant mice and examination of intestine, liver, spleen, and brain for lesions. Nine isolates infected only intestine; four isolates infected intestine and liver; one isolate infected intestine, liver, and brain; and one isolate infected liver. These results confirm that the enterotropic MHV biotype is predominant in contemporary laboratory mouse colonies. The MHV biotype features need to be taken into consideration when dealing with MHV infections.

Original languageEnglish (US)
Pages (from-to)50-54
Number of pages5
JournalLaboratory Animal Science
Volume48
Issue number1
StatePublished - 1998

Fingerprint

Murine hepatitis virus
Viruses
mice
biotypes
Liver
Intestines
intestines
liver
Virus Diseases
Brain
infection
tissue tropism
brain
nucleoproteins
Nucleoproteins
Tropism
prototypes
lesions (animal)
Virulence
spleen

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Animal Science and Zoology
  • veterinary(all)

Cite this

Prevalence of Enterotropic and Polytropic Mouse Hepatitis Virus in Enzootically Infected Mouse Colonies. / Homberger, Felix R.; Zhang, Linong; Barthold, Stephen W.

In: Laboratory Animal Science, Vol. 48, No. 1, 1998, p. 50-54.

Research output: Contribution to journalArticle

@article{40e7563b306f4d92ad6ddce4b19a0de8,
title = "Prevalence of Enterotropic and Polytropic Mouse Hepatitis Virus in Enzootically Infected Mouse Colonies",
abstract = "Mouse hepatitis virus (MHV) causes the most prevalent viral infection in contemporary laboratory mouse colonies. According to their primary replication site, different strains of MHV segregate into two overlapping biotypes, enterotropic and polytropic. These two groups vary greatly in disease pattern, pathogenicity, immune response, and duration of infection. Historically, the polytropic MHV strains represent the extensively studied prototype strains that have minimal enterotropism, whereas enterotropic MHV strains have been less characterized. Anecdotal reports suggest that most MHV strains encountered today belong to the enterotropic biotype. We have identified 15 isolates of MHV from 19 independent enzootically infected mouse colonies. Sequencing of a variable region of the nucleoprotein (N) gene of each isolate confirmed that all were independent genetic variants. The principal tissue tropism of the new isolates was determined by experimental inoculation of infant mice and examination of intestine, liver, spleen, and brain for lesions. Nine isolates infected only intestine; four isolates infected intestine and liver; one isolate infected intestine, liver, and brain; and one isolate infected liver. These results confirm that the enterotropic MHV biotype is predominant in contemporary laboratory mouse colonies. The MHV biotype features need to be taken into consideration when dealing with MHV infections.",
author = "Homberger, {Felix R.} and Linong Zhang and Barthold, {Stephen W}",
year = "1998",
language = "English (US)",
volume = "48",
pages = "50--54",
journal = "Comparative Medicine",
issn = "1532-0820",
publisher = "American Association for Laboratory Animal Science",
number = "1",

}

TY - JOUR

T1 - Prevalence of Enterotropic and Polytropic Mouse Hepatitis Virus in Enzootically Infected Mouse Colonies

AU - Homberger, Felix R.

AU - Zhang, Linong

AU - Barthold, Stephen W

PY - 1998

Y1 - 1998

N2 - Mouse hepatitis virus (MHV) causes the most prevalent viral infection in contemporary laboratory mouse colonies. According to their primary replication site, different strains of MHV segregate into two overlapping biotypes, enterotropic and polytropic. These two groups vary greatly in disease pattern, pathogenicity, immune response, and duration of infection. Historically, the polytropic MHV strains represent the extensively studied prototype strains that have minimal enterotropism, whereas enterotropic MHV strains have been less characterized. Anecdotal reports suggest that most MHV strains encountered today belong to the enterotropic biotype. We have identified 15 isolates of MHV from 19 independent enzootically infected mouse colonies. Sequencing of a variable region of the nucleoprotein (N) gene of each isolate confirmed that all were independent genetic variants. The principal tissue tropism of the new isolates was determined by experimental inoculation of infant mice and examination of intestine, liver, spleen, and brain for lesions. Nine isolates infected only intestine; four isolates infected intestine and liver; one isolate infected intestine, liver, and brain; and one isolate infected liver. These results confirm that the enterotropic MHV biotype is predominant in contemporary laboratory mouse colonies. The MHV biotype features need to be taken into consideration when dealing with MHV infections.

AB - Mouse hepatitis virus (MHV) causes the most prevalent viral infection in contemporary laboratory mouse colonies. According to their primary replication site, different strains of MHV segregate into two overlapping biotypes, enterotropic and polytropic. These two groups vary greatly in disease pattern, pathogenicity, immune response, and duration of infection. Historically, the polytropic MHV strains represent the extensively studied prototype strains that have minimal enterotropism, whereas enterotropic MHV strains have been less characterized. Anecdotal reports suggest that most MHV strains encountered today belong to the enterotropic biotype. We have identified 15 isolates of MHV from 19 independent enzootically infected mouse colonies. Sequencing of a variable region of the nucleoprotein (N) gene of each isolate confirmed that all were independent genetic variants. The principal tissue tropism of the new isolates was determined by experimental inoculation of infant mice and examination of intestine, liver, spleen, and brain for lesions. Nine isolates infected only intestine; four isolates infected intestine and liver; one isolate infected intestine, liver, and brain; and one isolate infected liver. These results confirm that the enterotropic MHV biotype is predominant in contemporary laboratory mouse colonies. The MHV biotype features need to be taken into consideration when dealing with MHV infections.

UR - http://www.scopus.com/inward/record.url?scp=0031989142&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031989142&partnerID=8YFLogxK

M3 - Article

C2 - 9517890

AN - SCOPUS:0031989142

VL - 48

SP - 50

EP - 54

JO - Comparative Medicine

JF - Comparative Medicine

SN - 1532-0820

IS - 1

ER -