Prevalence of bluetongue virus expression in leukocytes from experimentally infected ruminants.

J. A. Ellis, M. L. Coen, Nigel J Maclachlan, W. C. Wilson, E. S. Williams, A. J. Leudke

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Replication of bluetongue virus (BTV) in leukocytes from the blood of sheep, cattle, elk, and mule deer inoculated with BTV serotype 10 or 17 was assessed by immunocytochemical staining and dot blot northern hybridization to determine if differences in the prevalence of infection in this blood fraction might account for the differences in clinical disease among these species. Viremia was confirmed by virus isolation in all inoculated animals. Analysis of leukocytes with monoclonal antibodies specific for BTV proteins revealed low numbers of infected leukocytes in only 2 sheep 8 days after inoculation with BTV serotype 10. Most of the cells expressing BTV were identified morphologically as monocytes; approximately 10% of infected cells were lymphocytes. Bluetongue virus was not detected by use of dot-blot hybridization on samples of blood. Our results suggest that differential infection of leukocytes does not account for the pronounced differences in clinical signs and pathologic changes among ruminants.

Original languageEnglish (US)
Pages (from-to)1452-1456
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume54
Issue number9
StatePublished - Sep 1993
Externally publishedYes

Fingerprint

Bluetongue virus
Ruminants
ruminants
leukocytes
Leukocytes
Sheep
blood
serotypes
sheep
Odocoileus hemionus
Deer
Equidae
elks
Viremia
viremia
Infection
nucleic acid hybridization
Leukocyte Count
leukocyte count
Northern blotting

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Ellis, J. A., Coen, M. L., Maclachlan, N. J., Wilson, W. C., Williams, E. S., & Leudke, A. J. (1993). Prevalence of bluetongue virus expression in leukocytes from experimentally infected ruminants. American Journal of Veterinary Research, 54(9), 1452-1456.

Prevalence of bluetongue virus expression in leukocytes from experimentally infected ruminants. / Ellis, J. A.; Coen, M. L.; Maclachlan, Nigel J; Wilson, W. C.; Williams, E. S.; Leudke, A. J.

In: American Journal of Veterinary Research, Vol. 54, No. 9, 09.1993, p. 1452-1456.

Research output: Contribution to journalArticle

Ellis, JA, Coen, ML, Maclachlan, NJ, Wilson, WC, Williams, ES & Leudke, AJ 1993, 'Prevalence of bluetongue virus expression in leukocytes from experimentally infected ruminants.', American Journal of Veterinary Research, vol. 54, no. 9, pp. 1452-1456.
Ellis, J. A. ; Coen, M. L. ; Maclachlan, Nigel J ; Wilson, W. C. ; Williams, E. S. ; Leudke, A. J. / Prevalence of bluetongue virus expression in leukocytes from experimentally infected ruminants. In: American Journal of Veterinary Research. 1993 ; Vol. 54, No. 9. pp. 1452-1456.
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