Abstract
Objectives: Bartonella infection has been associated with endocarditis in humans, dogs, cats and cattle. In order to evaluate the importance of this pathogen as a possible source of endocarditis in United States military working dogs (MWDs), we performed a retrospective case–control study on 26 dogs with histological diagnosis of culture negative endocarditis (n = 18), endomyocarditis (n = 5) or endocardiosis (n = 3) and 28 control dogs without any histological cardiac lesions. Methods: DNA was extracted from paraffin embedded cardiac valves and tissues from case and control dogs and submitted to PCR testing with primers targeting the Bartonella gltA gene. PCR-RFLP using four restriction endonucleases and partial sequencing was then performed to determine the Bartonella species involved. Results: Nineteen (73%) cases were PCR positive for Bartonella, including B. henselae (8 dogs), B. vinsonii subsp. berkhoffii (6 dogs), B. washoensis (2 dogs) and B. elizabethae (1 dog). Only one control dog was weakly PCR positive for Bartonella. Based on the type of histological diagnosis, 13 (72.2%) dogs with endocarditis, 3 (60%) dogs with endomyocarditis and all 3 dogs with endocardiosis were Bartonella PCR positive. Conclusions: Bartonella sp. Infections were correlated with cardiopathies in US military working dogs. Systemic use of insecticides against ectoparasites and regular testing of MWDs for Bartonella infection seem highly appropriate to prevent such life-threatening exposures.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-9 |
| Number of pages | 9 |
| Journal | Journal of Veterinary Cardiology |
| Volume | 27 |
| DOIs | |
| State | Published - Feb 2020 |
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Keywords
- Bartonella sp.
- Canids
- Non-culturable cardiac infection
- USA
ASJC Scopus subject areas
- Physiology
- veterinary(all)
Cite this
Prevalence of Bartonella sp. in United States military working dogs with infectious endocarditis : a retrospective case–control study. / Davis, A. Z.; Jaffe, D. A.; Honadel, T. E.; Lapsley, W. D.; Wilber-Raymond, J. L.; Kasten, R. W.; Chomel, B. B.
In: Journal of Veterinary Cardiology, Vol. 27, 02.2020, p. 1-9.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prevalence of Bartonella sp. in United States military working dogs with infectious endocarditis
T2 - a retrospective case–control study
AU - Davis, A. Z.
AU - Jaffe, D. A.
AU - Honadel, T. E.
AU - Lapsley, W. D.
AU - Wilber-Raymond, J. L.
AU - Kasten, R. W.
AU - Chomel, B. B.
PY - 2020/2
Y1 - 2020/2
N2 - Objectives: Bartonella infection has been associated with endocarditis in humans, dogs, cats and cattle. In order to evaluate the importance of this pathogen as a possible source of endocarditis in United States military working dogs (MWDs), we performed a retrospective case–control study on 26 dogs with histological diagnosis of culture negative endocarditis (n = 18), endomyocarditis (n = 5) or endocardiosis (n = 3) and 28 control dogs without any histological cardiac lesions. Methods: DNA was extracted from paraffin embedded cardiac valves and tissues from case and control dogs and submitted to PCR testing with primers targeting the Bartonella gltA gene. PCR-RFLP using four restriction endonucleases and partial sequencing was then performed to determine the Bartonella species involved. Results: Nineteen (73%) cases were PCR positive for Bartonella, including B. henselae (8 dogs), B. vinsonii subsp. berkhoffii (6 dogs), B. washoensis (2 dogs) and B. elizabethae (1 dog). Only one control dog was weakly PCR positive for Bartonella. Based on the type of histological diagnosis, 13 (72.2%) dogs with endocarditis, 3 (60%) dogs with endomyocarditis and all 3 dogs with endocardiosis were Bartonella PCR positive. Conclusions: Bartonella sp. Infections were correlated with cardiopathies in US military working dogs. Systemic use of insecticides against ectoparasites and regular testing of MWDs for Bartonella infection seem highly appropriate to prevent such life-threatening exposures.
AB - Objectives: Bartonella infection has been associated with endocarditis in humans, dogs, cats and cattle. In order to evaluate the importance of this pathogen as a possible source of endocarditis in United States military working dogs (MWDs), we performed a retrospective case–control study on 26 dogs with histological diagnosis of culture negative endocarditis (n = 18), endomyocarditis (n = 5) or endocardiosis (n = 3) and 28 control dogs without any histological cardiac lesions. Methods: DNA was extracted from paraffin embedded cardiac valves and tissues from case and control dogs and submitted to PCR testing with primers targeting the Bartonella gltA gene. PCR-RFLP using four restriction endonucleases and partial sequencing was then performed to determine the Bartonella species involved. Results: Nineteen (73%) cases were PCR positive for Bartonella, including B. henselae (8 dogs), B. vinsonii subsp. berkhoffii (6 dogs), B. washoensis (2 dogs) and B. elizabethae (1 dog). Only one control dog was weakly PCR positive for Bartonella. Based on the type of histological diagnosis, 13 (72.2%) dogs with endocarditis, 3 (60%) dogs with endomyocarditis and all 3 dogs with endocardiosis were Bartonella PCR positive. Conclusions: Bartonella sp. Infections were correlated with cardiopathies in US military working dogs. Systemic use of insecticides against ectoparasites and regular testing of MWDs for Bartonella infection seem highly appropriate to prevent such life-threatening exposures.
KW - Bartonella sp.
KW - Canids
KW - Non-culturable cardiac infection
KW - USA
UR - http://www.scopus.com/inward/record.url?scp=85076003867&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85076003867&partnerID=8YFLogxK
U2 - 10.1016/j.jvc.2019.11.005
DO - 10.1016/j.jvc.2019.11.005
M3 - Article
C2 - 31830708
AN - SCOPUS:85076003867
VL - 27
SP - 1
EP - 9
JO - Journal of Veterinary Cardiology
JF - Journal of Veterinary Cardiology
SN - 1760-2734
ER -