Prevalence of Bartonella sp. in United States military working dogs with infectious endocarditis: a retrospective case–control study

A. Z. Davis, D. A. Jaffe, T. E. Honadel, W. D. Lapsley, J. L. Wilber-Raymond, R. W. Kasten, B. B. Chomel

Research output: Contribution to journalArticle

Abstract

Objectives: Bartonella infection has been associated with endocarditis in humans, dogs, cats and cattle. In order to evaluate the importance of this pathogen as a possible source of endocarditis in United States military working dogs (MWDs), we performed a retrospective case–control study on 26 dogs with histological diagnosis of culture negative endocarditis (n = 18), endomyocarditis (n = 5) or endocardiosis (n = 3) and 28 control dogs without any histological cardiac lesions. Methods: DNA was extracted from paraffin embedded cardiac valves and tissues from case and control dogs and submitted to PCR testing with primers targeting the Bartonella gltA gene. PCR-RFLP using four restriction endonucleases and partial sequencing was then performed to determine the Bartonella species involved. Results: Nineteen (73%) cases were PCR positive for Bartonella, including B. henselae (8 dogs), B. vinsonii subsp. berkhoffii (6 dogs), B. washoensis (2 dogs) and B. elizabethae (1 dog). Only one control dog was weakly PCR positive for Bartonella. Based on the type of histological diagnosis, 13 (72.2%) dogs with endocarditis, 3 (60%) dogs with endomyocarditis and all 3 dogs with endocardiosis were Bartonella PCR positive. Conclusions: Bartonella sp. Infections were correlated with cardiopathies in US military working dogs. Systemic use of insecticides against ectoparasites and regular testing of MWDs for Bartonella infection seem highly appropriate to prevent such life-threatening exposures.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalJournal of Veterinary Cardiology
Volume27
DOIs
StatePublished - Feb 2020

Fingerprint

Bartonella
endocarditis
Endocarditis
retrospective studies
Retrospective Studies
Dogs
dogs
Polymerase Chain Reaction
Bartonella Infections
Bartonella elizabethae
Bartonella vinsonii subsp. berkhoffii
infection
Bartonella henselae
Heart Valves
DNA Restriction Enzymes
restriction endonucleases

Keywords

  • Bartonella sp.
  • Canids
  • Non-culturable cardiac infection
  • USA

ASJC Scopus subject areas

  • Physiology
  • veterinary(all)

Cite this

Prevalence of Bartonella sp. in United States military working dogs with infectious endocarditis : a retrospective case–control study. / Davis, A. Z.; Jaffe, D. A.; Honadel, T. E.; Lapsley, W. D.; Wilber-Raymond, J. L.; Kasten, R. W.; Chomel, B. B.

In: Journal of Veterinary Cardiology, Vol. 27, 02.2020, p. 1-9.

Research output: Contribution to journalArticle

Davis, A. Z. ; Jaffe, D. A. ; Honadel, T. E. ; Lapsley, W. D. ; Wilber-Raymond, J. L. ; Kasten, R. W. ; Chomel, B. B. / Prevalence of Bartonella sp. in United States military working dogs with infectious endocarditis : a retrospective case–control study. In: Journal of Veterinary Cardiology. 2020 ; Vol. 27. pp. 1-9.
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abstract = "Objectives: Bartonella infection has been associated with endocarditis in humans, dogs, cats and cattle. In order to evaluate the importance of this pathogen as a possible source of endocarditis in United States military working dogs (MWDs), we performed a retrospective case–control study on 26 dogs with histological diagnosis of culture negative endocarditis (n = 18), endomyocarditis (n = 5) or endocardiosis (n = 3) and 28 control dogs without any histological cardiac lesions. Methods: DNA was extracted from paraffin embedded cardiac valves and tissues from case and control dogs and submitted to PCR testing with primers targeting the Bartonella gltA gene. PCR-RFLP using four restriction endonucleases and partial sequencing was then performed to determine the Bartonella species involved. Results: Nineteen (73{\%}) cases were PCR positive for Bartonella, including B. henselae (8 dogs), B. vinsonii subsp. berkhoffii (6 dogs), B. washoensis (2 dogs) and B. elizabethae (1 dog). Only one control dog was weakly PCR positive for Bartonella. Based on the type of histological diagnosis, 13 (72.2{\%}) dogs with endocarditis, 3 (60{\%}) dogs with endomyocarditis and all 3 dogs with endocardiosis were Bartonella PCR positive. Conclusions: Bartonella sp. Infections were correlated with cardiopathies in US military working dogs. Systemic use of insecticides against ectoparasites and regular testing of MWDs for Bartonella infection seem highly appropriate to prevent such life-threatening exposures.",
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