Prevalence and molecular characterization of Escherichia coli O157

H7 by multiple-locus variable-number tandem repeat analysis and pulsed-field gel electrophoresis in three sheep farming operations in California

Christopher Kilonzo, Edward R Atwill, Robert Mandrell, Melissa Garrick, Veronica Villanueva, Bruce R. Hoar

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A year-long study was conducted to determine the fecal prevalence of Escherichia coli O157:H7 in three sheep ranches. Strain diversity and persistence were compared with multiple-locus variable-number tandem repeat analysis and pulsed-field gel electrophoresis. Ranch C, a feedlot, consisted of young sheep raised predominantly on a high-grain diet. The other two sites consisted of sheep raised on native pasture and a combination of native and irrigated pasture. Forty fecal samples were collected every month from each ranch. Samples were examined for E. coli O157:H7 by immunomagnetic separation and culture of the magnetic beads onto selective media. Detection of virulence markers in positive isolates was determined by PCR. E. coli O157:H7 was isolated from 100 (22.7%) of 440 fecal samples collected from ranch C. On ranch B, 9 (1.9%) of the 480 fecal samples were positive for the pathogen, while none of the samples from ranch A were positive. On ranch C, the odds of detecting E. coli O157:H7 was 3.2 times greater during the warmer months compared with the cooler months of the year. There was no association between days spent in the feedlot and fecal prevalence of the pathogen (P ~ 0.62). Most multiple-locus variablenumber tandem repeat analysis types were isolated only once from ranch C (14 of 23), but several strains were isolated over 4 to 6 months, often in many intervening negative months. This study revealed that the prevalence of E. coli O157:H7 can be high in some sheep ranches in California, especially in feedlots where young sheep are fed predominantly high-grain rations.

Original languageEnglish (US)
Pages (from-to)1413-1421
Number of pages9
JournalJournal of Food Protection
Volume74
Issue number9
DOIs
StatePublished - Sep 2011

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Minisatellite Repeats
Escherichia coli O157
Pulsed Field Gel Electrophoresis
ranching
pulsed-field gel electrophoresis
Agriculture
Sheep
farming systems
sheep
feedlots
Immunomagnetic Separation
Tandem Repeat Sequences
sampling
Virulence
pastures
Cross-Sectional Studies
multiple-locus variable number tandem-repeat analysis
immunomagnetic separation
Diet
tandem repeat sequences

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Prevalence and molecular characterization of Escherichia coli O157 : H7 by multiple-locus variable-number tandem repeat analysis and pulsed-field gel electrophoresis in three sheep farming operations in California. / Kilonzo, Christopher; Atwill, Edward R; Mandrell, Robert; Garrick, Melissa; Villanueva, Veronica; Hoar, Bruce R.

In: Journal of Food Protection, Vol. 74, No. 9, 09.2011, p. 1413-1421.

Research output: Contribution to journalArticle

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abstract = "A year-long study was conducted to determine the fecal prevalence of Escherichia coli O157:H7 in three sheep ranches. Strain diversity and persistence were compared with multiple-locus variable-number tandem repeat analysis and pulsed-field gel electrophoresis. Ranch C, a feedlot, consisted of young sheep raised predominantly on a high-grain diet. The other two sites consisted of sheep raised on native pasture and a combination of native and irrigated pasture. Forty fecal samples were collected every month from each ranch. Samples were examined for E. coli O157:H7 by immunomagnetic separation and culture of the magnetic beads onto selective media. Detection of virulence markers in positive isolates was determined by PCR. E. coli O157:H7 was isolated from 100 (22.7{\%}) of 440 fecal samples collected from ranch C. On ranch B, 9 (1.9{\%}) of the 480 fecal samples were positive for the pathogen, while none of the samples from ranch A were positive. On ranch C, the odds of detecting E. coli O157:H7 was 3.2 times greater during the warmer months compared with the cooler months of the year. There was no association between days spent in the feedlot and fecal prevalence of the pathogen (P ~ 0.62). Most multiple-locus variablenumber tandem repeat analysis types were isolated only once from ranch C (14 of 23), but several strains were isolated over 4 to 6 months, often in many intervening negative months. This study revealed that the prevalence of E. coli O157:H7 can be high in some sheep ranches in California, especially in feedlots where young sheep are fed predominantly high-grain rations.",
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