Pretargeted immunoscintigraphy: Effect of hapten valency on murine tumor uptake

D. A. Goodwin, C. F. Meares, M. McTigue, W. Chaovapong, C. I. Diamanti, C. H. Ransone, M. J. McCall

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

A method of radioimmunoscintigraphy using bivalent 'Janus' haptens with an apparent enhanced affinity ('avidity') for the antibody is described. Janus with 50 μg pretargeted Mab WC3A11 resulted in significantly higher murine tumor concentrations (~7%/g) compared to monovalent haptens (~1.4%/g, p < 0.001), and the same high tumor-to-background ratios (~3/1). Janus was synthesized by coupling two molecules of BABE together with a 1,4 butanedithiol linker. Janus itself was rapidly excreted (T( 1/2 (b)) = 42 min) by the kidneys and did not concentrate in any other organs or tissues. Three- step pretargeted immunoscintigraphy (binder, chaser, tracer) with 111In- or 67Ga-Co(III) Janus produced excellent mouse tumor images in 3 hr with high tumor-to-background ratios. The use of short-lived tracers, such as 99mTc and 68Ga, with a T( 1/2 p) of hours to image antibodies that localize slowly over several days in vivo is accessible with this new technology.

Original languageEnglish (US)
Pages (from-to)2006-2013
Number of pages8
JournalJournal of Nuclear Medicine
Volume33
Issue number11
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Fingerprint Dive into the research topics of 'Pretargeted immunoscintigraphy: Effect of hapten valency on murine tumor uptake'. Together they form a unique fingerprint.

  • Cite this

    Goodwin, D. A., Meares, C. F., McTigue, M., Chaovapong, W., Diamanti, C. I., Ransone, C. H., & McCall, M. J. (1992). Pretargeted immunoscintigraphy: Effect of hapten valency on murine tumor uptake. Journal of Nuclear Medicine, 33(11), 2006-2013.