Presynaptic mitochondrial morphology in monkey prefrontal cortex correlates with working memory and is improved with estrogen treatment

Yuko Hara, Frank Yuk, Rishi Puri, William G.M. Janssen, Peter R. Rapp, John Morrison

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Humans and nonhuman primates are vulnerable to age- and menopause-related decline in working memory, a cognitive function reliant on the energy-demanding recurrent excitation of neurons within Brodmann's Area 46 of the dorsolateral prefrontal cortex (dlPFC). Here, we tested the hypothesis that the number and morphology (straight, curved, or donut-shaped) of mitochondria in dlPFC presynaptic boutons are altered with aging and menopause in rhesus monkeys (Macaca mulatta) and that these metrics correlate with delayed response (DR) accuracy, a well-characterized measure of dlPFC-dependent working memory. Although presynaptic bouton density or size was not significantly different across groups distinguished by age or menses status, DR accuracy correlated positively with the number of total and straight mitochondria per dlPFC bouton. In contrast, DR accuracy correlated inversely with the frequency of boutons containing donut-shaped mitochondria, which exhibited smaller active zone areas and fewer docked synaptic vesicles than those with straight or curved mitochondria. We then examined the effects of estrogen administration to testwhether a treatment known to improve workingmemory influences mitochondrial morphology. Aged ovariectomized monkeys treated with vehicle displayed significant working memory impairment and a concomitant 44% increase in presynaptic donutshaped mitochondria, both of which were reversed with cyclic estradiol treatment. Together, our data suggest that hormone replacement therapy may benefit cognitive aging, in part by promoting mitochondrial and synaptic health in the dlPFC.

Original languageEnglish (US)
Pages (from-to)486-491
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number1
DOIs
StatePublished - Jan 16 2014
Externally publishedYes

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Prefrontal Cortex
Short-Term Memory
Haplorhini
Mitochondria
Estrogens
Menopause
Macaca mulatta
Therapeutics
Menstruation
Synaptic Vesicles
Hormone Replacement Therapy
Cognition
Primates
Estradiol
Age Groups
Neurons
Health

Keywords

  • Axonal bouton
  • Cognition
  • Toroidal mitochondria

ASJC Scopus subject areas

  • General

Cite this

Presynaptic mitochondrial morphology in monkey prefrontal cortex correlates with working memory and is improved with estrogen treatment. / Hara, Yuko; Yuk, Frank; Puri, Rishi; Janssen, William G.M.; Rapp, Peter R.; Morrison, John.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 1, 16.01.2014, p. 486-491.

Research output: Contribution to journalArticle

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