Presentation of autoantigen by human T cells

Janine M LaSalle, Kohei Ota, David A. Hafler

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Activated human T cells express MHC class II and have been shown to present foreign Ag to autologous T cells. We now demonstrate that MHC class II+ T cell clones can present myelin basic protein (MBP) peptide autoantigen in the absence of traditional APC to autologous MBP reactive T cell clones. MBP peptide-pulsed T cell clones specifically stimulated autologous MBP-reactive T cell clones to flux calcium and proliferate. Activation responses were peptide epitope specific and blocked by mAb to MHC class II, indicating a TCR-mediated response. In addition, mAb to the adhesion molecules LFA-3, CD2, LFA-1, CD29, and to the tyrosine phosphatase CD45 also inhibited proliferation, indicating the involvement of T to T cell interactions. In contrast to peptide Ag, T cell clones did not respond to autologous T cells pulsed with HPLC-purified MBP, suggesting that T cells are unable to process whole MBP. However, batch-purified MBP Ag preparations containing lower m.w. breakdown products were presented by T cells, indicating that naturally occurring breakdown products of autoantigens could be presented by activated T cells in vivo. These results raise the possibility that T cell presentation of autoantigen at inflammatory sites may be important in regulation of immune responses to self Ag.

Original languageEnglish (US)
Pages (from-to)774-780
Number of pages7
JournalJournal of Immunology
Volume147
Issue number3
StatePublished - Aug 1 1991
Externally publishedYes

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Autoantigens
T-Lymphocytes
Myelin Basic Protein
Clone Cells
Peptide T
CD58 Antigens
Lymphocyte Function-Associated Antigen-1
Peptides
Phosphoric Monoester Hydrolases
Cell Communication
Tyrosine
Epitopes
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Immunology

Cite this

LaSalle, J. M., Ota, K., & Hafler, D. A. (1991). Presentation of autoantigen by human T cells. Journal of Immunology, 147(3), 774-780.

Presentation of autoantigen by human T cells. / LaSalle, Janine M; Ota, Kohei; Hafler, David A.

In: Journal of Immunology, Vol. 147, No. 3, 01.08.1991, p. 774-780.

Research output: Contribution to journalArticle

LaSalle, JM, Ota, K & Hafler, DA 1991, 'Presentation of autoantigen by human T cells', Journal of Immunology, vol. 147, no. 3, pp. 774-780.
LaSalle JM, Ota K, Hafler DA. Presentation of autoantigen by human T cells. Journal of Immunology. 1991 Aug 1;147(3):774-780.
LaSalle, Janine M ; Ota, Kohei ; Hafler, David A. / Presentation of autoantigen by human T cells. In: Journal of Immunology. 1991 ; Vol. 147, No. 3. pp. 774-780.
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