Abstract
MCF-7 human mammary epithelial cells are known to express three distinct cytoskeletal intermediate filament proteins designated numbers 8 (Mr52,000), 18 (Mr45,000), and 19 (Mr40,000) keratin. Using a panel of monoclonal antibodies and peptide mapping, we have determined that MCF-7 cells release proteolytic derivatives of keratins 18 and 19 into the culture medium. These extracellular keratins consist of multiple isoforms (Mr38,000-45,000, isoelectric point 5.0-5.2) on two-dimen-sional gels, many of which are smaller and more acidic than the predominant intracellular forms and appear to be associated together as a soluble, large molecular weight complex. These results may potentially be correlated with observations of keratin-like antigens in body fluids of cancer patients and the presence of antikeratin antibodies in individuals with certain autoimmune diseases and acute viral infections.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6353-6359 |
| Number of pages | 7 |
| Journal | Cancer Research |
| Volume | 46 |
| State | Published - Jan 1 1986 |
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ASJC Scopus subject areas
- Oncology
- Cancer Research
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Presence of proteolytically processed keratins in the culture medium of MCF-7 cells. / Chan, Raymond; Rossitto, Paul V.; Edwards, Benjamin F.; Cardiff, Robert.
In: Cancer Research, Vol. 46, 01.01.1986, p. 6353-6359.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Presence of proteolytically processed keratins in the culture medium of MCF-7 cells
AU - Chan, Raymond
AU - Rossitto, Paul V.
AU - Edwards, Benjamin F.
AU - Cardiff, Robert
PY - 1986/1/1
Y1 - 1986/1/1
N2 - MCF-7 human mammary epithelial cells are known to express three distinct cytoskeletal intermediate filament proteins designated numbers 8 (Mr52,000), 18 (Mr45,000), and 19 (Mr40,000) keratin. Using a panel of monoclonal antibodies and peptide mapping, we have determined that MCF-7 cells release proteolytic derivatives of keratins 18 and 19 into the culture medium. These extracellular keratins consist of multiple isoforms (Mr38,000-45,000, isoelectric point 5.0-5.2) on two-dimen-sional gels, many of which are smaller and more acidic than the predominant intracellular forms and appear to be associated together as a soluble, large molecular weight complex. These results may potentially be correlated with observations of keratin-like antigens in body fluids of cancer patients and the presence of antikeratin antibodies in individuals with certain autoimmune diseases and acute viral infections.
AB - MCF-7 human mammary epithelial cells are known to express three distinct cytoskeletal intermediate filament proteins designated numbers 8 (Mr52,000), 18 (Mr45,000), and 19 (Mr40,000) keratin. Using a panel of monoclonal antibodies and peptide mapping, we have determined that MCF-7 cells release proteolytic derivatives of keratins 18 and 19 into the culture medium. These extracellular keratins consist of multiple isoforms (Mr38,000-45,000, isoelectric point 5.0-5.2) on two-dimen-sional gels, many of which are smaller and more acidic than the predominant intracellular forms and appear to be associated together as a soluble, large molecular weight complex. These results may potentially be correlated with observations of keratin-like antigens in body fluids of cancer patients and the presence of antikeratin antibodies in individuals with certain autoimmune diseases and acute viral infections.
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M3 - Article
C2 - 2430694
AN - SCOPUS:0022826715
VL - 46
SP - 6353
EP - 6359
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0099-7013
ER -