Presence of proteolytically processed keratins in the culture medium of MCF-7 cells

Raymond Chan, Paul V. Rossitto, Benjamin F. Edwards, Robert Cardiff

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

MCF-7 human mammary epithelial cells are known to express three distinct cytoskeletal intermediate filament proteins designated numbers 8 (Mr52,000), 18 (Mr45,000), and 19 (Mr40,000) keratin. Using a panel of monoclonal antibodies and peptide mapping, we have determined that MCF-7 cells release proteolytic derivatives of keratins 18 and 19 into the culture medium. These extracellular keratins consist of multiple isoforms (Mr38,000-45,000, isoelectric point 5.0-5.2) on two-dimen-sional gels, many of which are smaller and more acidic than the predominant intracellular forms and appear to be associated together as a soluble, large molecular weight complex. These results may potentially be correlated with observations of keratin-like antigens in body fluids of cancer patients and the presence of antikeratin antibodies in individuals with certain autoimmune diseases and acute viral infections.

Original languageEnglish (US)
Pages (from-to)6353-6359
Number of pages7
JournalCancer Research
Volume46
StatePublished - Jan 1 1986

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MCF-7 Cells
Keratins
Culture Media
Keratin-18
Keratin-19
Intermediate Filament Proteins
Peptide Mapping
Cytoskeletal Proteins
Isoelectric Point
Body Fluids
Virus Diseases
Cytoskeleton
Autoimmune Diseases
Protein Isoforms
Breast
Molecular Weight
Gels
Epithelial Cells
Monoclonal Antibodies
Antigens

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Presence of proteolytically processed keratins in the culture medium of MCF-7 cells. / Chan, Raymond; Rossitto, Paul V.; Edwards, Benjamin F.; Cardiff, Robert.

In: Cancer Research, Vol. 46, 01.01.1986, p. 6353-6359.

Research output: Contribution to journalArticle

Chan, Raymond ; Rossitto, Paul V. ; Edwards, Benjamin F. ; Cardiff, Robert. / Presence of proteolytically processed keratins in the culture medium of MCF-7 cells. In: Cancer Research. 1986 ; Vol. 46. pp. 6353-6359.
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