Preparation of synaptic vesicles from mammalian brain

Johannes W Hell, Reinhard Jahn

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

This chapter describes procedures for preparing synaptic vesicles from mammalian brain. Synaptic vesicles possess several remarkable properties that distinguish them from most other organelles involved in membrane traffic. First, they are very abundant in brain tissue. Synaptic vesicles are highly homogeneous in size and shape and, in addition, are smaller than most other organelles, with an average diameter of only 50 nm. Therefore, size-fractionation techniques can be applied for the isolation of synaptic vesicles. The procedure for preparing synaptic vesicles from frozen brain starts with a harsh homogenization of frozen brains to efficiently break up the nerve terminals, thus releasing synaptic vesicles. Frozen brains are ground in a pre-cooled mortar to yield a fine powder. To create a tissue powder, place the frozen brains in a porcelain mortar pre-cooled with liquid nitrogen. Cover them with cheesecloth and break them carefully using a porcelain pestle. Synaptic vesicle membranes are identified by their very uniform appearance. © 2006

Original languageEnglish (US)
Title of host publicationCell Biology, Four-Volume Set
PublisherElsevier Inc.
Pages85-90
Number of pages6
Volume2
ISBN (Print)9780121647308
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Synaptic Vesicles
Brain
Dental Porcelain
Mortar
Powders
Organelles
Tissue
Membranes
Synaptic Membranes
Liquid nitrogen
Fractionation
Nitrogen

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Hell, J. W., & Jahn, R. (2006). Preparation of synaptic vesicles from mammalian brain. In Cell Biology, Four-Volume Set (Vol. 2, pp. 85-90). Elsevier Inc.. https://doi.org/10.1016/B978-012164730-8/50084-8

Preparation of synaptic vesicles from mammalian brain. / Hell, Johannes W; Jahn, Reinhard.

Cell Biology, Four-Volume Set. Vol. 2 Elsevier Inc., 2006. p. 85-90.

Research output: Chapter in Book/Report/Conference proceedingChapter

Hell, JW & Jahn, R 2006, Preparation of synaptic vesicles from mammalian brain. in Cell Biology, Four-Volume Set. vol. 2, Elsevier Inc., pp. 85-90. https://doi.org/10.1016/B978-012164730-8/50084-8
Hell JW, Jahn R. Preparation of synaptic vesicles from mammalian brain. In Cell Biology, Four-Volume Set. Vol. 2. Elsevier Inc. 2006. p. 85-90 https://doi.org/10.1016/B978-012164730-8/50084-8
Hell, Johannes W ; Jahn, Reinhard. / Preparation of synaptic vesicles from mammalian brain. Cell Biology, Four-Volume Set. Vol. 2 Elsevier Inc., 2006. pp. 85-90
@inbook{bd35f02234154e7cb27116cc45162621,
title = "Preparation of synaptic vesicles from mammalian brain",
abstract = "This chapter describes procedures for preparing synaptic vesicles from mammalian brain. Synaptic vesicles possess several remarkable properties that distinguish them from most other organelles involved in membrane traffic. First, they are very abundant in brain tissue. Synaptic vesicles are highly homogeneous in size and shape and, in addition, are smaller than most other organelles, with an average diameter of only 50 nm. Therefore, size-fractionation techniques can be applied for the isolation of synaptic vesicles. The procedure for preparing synaptic vesicles from frozen brain starts with a harsh homogenization of frozen brains to efficiently break up the nerve terminals, thus releasing synaptic vesicles. Frozen brains are ground in a pre-cooled mortar to yield a fine powder. To create a tissue powder, place the frozen brains in a porcelain mortar pre-cooled with liquid nitrogen. Cover them with cheesecloth and break them carefully using a porcelain pestle. Synaptic vesicle membranes are identified by their very uniform appearance. {\circledC} 2006",
author = "Hell, {Johannes W} and Reinhard Jahn",
year = "2006",
doi = "10.1016/B978-012164730-8/50084-8",
language = "English (US)",
isbn = "9780121647308",
volume = "2",
pages = "85--90",
booktitle = "Cell Biology, Four-Volume Set",
publisher = "Elsevier Inc.",

}

TY - CHAP

T1 - Preparation of synaptic vesicles from mammalian brain

AU - Hell, Johannes W

AU - Jahn, Reinhard

PY - 2006

Y1 - 2006

N2 - This chapter describes procedures for preparing synaptic vesicles from mammalian brain. Synaptic vesicles possess several remarkable properties that distinguish them from most other organelles involved in membrane traffic. First, they are very abundant in brain tissue. Synaptic vesicles are highly homogeneous in size and shape and, in addition, are smaller than most other organelles, with an average diameter of only 50 nm. Therefore, size-fractionation techniques can be applied for the isolation of synaptic vesicles. The procedure for preparing synaptic vesicles from frozen brain starts with a harsh homogenization of frozen brains to efficiently break up the nerve terminals, thus releasing synaptic vesicles. Frozen brains are ground in a pre-cooled mortar to yield a fine powder. To create a tissue powder, place the frozen brains in a porcelain mortar pre-cooled with liquid nitrogen. Cover them with cheesecloth and break them carefully using a porcelain pestle. Synaptic vesicle membranes are identified by their very uniform appearance. © 2006

AB - This chapter describes procedures for preparing synaptic vesicles from mammalian brain. Synaptic vesicles possess several remarkable properties that distinguish them from most other organelles involved in membrane traffic. First, they are very abundant in brain tissue. Synaptic vesicles are highly homogeneous in size and shape and, in addition, are smaller than most other organelles, with an average diameter of only 50 nm. Therefore, size-fractionation techniques can be applied for the isolation of synaptic vesicles. The procedure for preparing synaptic vesicles from frozen brain starts with a harsh homogenization of frozen brains to efficiently break up the nerve terminals, thus releasing synaptic vesicles. Frozen brains are ground in a pre-cooled mortar to yield a fine powder. To create a tissue powder, place the frozen brains in a porcelain mortar pre-cooled with liquid nitrogen. Cover them with cheesecloth and break them carefully using a porcelain pestle. Synaptic vesicle membranes are identified by their very uniform appearance. © 2006

UR - http://www.scopus.com/inward/record.url?scp=84884866095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884866095&partnerID=8YFLogxK

U2 - 10.1016/B978-012164730-8/50084-8

DO - 10.1016/B978-012164730-8/50084-8

M3 - Chapter

AN - SCOPUS:84884866095

SN - 9780121647308

VL - 2

SP - 85

EP - 90

BT - Cell Biology, Four-Volume Set

PB - Elsevier Inc.

ER -