Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola

Inge Van Bogaert; Guodong Zhang; Jun Yang; Jun Yan Liu; Yonghao Ye; Wim Soetaert; Bruce D. Hammock

doi:10.1194/jlr.D042226

Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola

Inge Van Bogaert, Guodong Zhang, Jun Yang, Jun Yan Liu, Yonghao Ye, Wim Soetaert, Bruce D. Hammock

Entomology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20- hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.

Original language	English (US)
Pages (from-to)	3215-3219
Number of pages	5
Journal	Journal of Lipid Research
Volume	54
Issue number	11
DOIs	https://doi.org/10.1194/jlr.D042226
State	Published - Nov 2013

Keywords

20-hydroxyeicosatetraenoic acid
Arachidonic acid
Sophorolipids

ASJC Scopus subject areas

Biochemistry
Cell Biology
Endocrinology

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title = "Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola",

abstract = "The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20- hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.",

keywords = "20-hydroxyeicosatetraenoic acid, Arachidonic acid, Sophorolipids",

author = "{Van Bogaert}, Inge and Guodong Zhang and Jun Yang and Liu, {Jun Yan} and Yonghao Ye and Wim Soetaert and Hammock, {Bruce D.}",

year = "2013",

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language = "English (US)",

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T1 - Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola

AU - Van Bogaert, Inge

AU - Zhang, Guodong

AU - Yang, Jun

AU - Liu, Jun Yan

AU - Ye, Yonghao

AU - Soetaert, Wim

AU - Hammock, Bruce D.

PY - 2013/11

Y1 - 2013/11

N2 - The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20- hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.

AB - The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20- hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.

KW - 20-hydroxyeicosatetraenoic acid

KW - Arachidonic acid

KW - Sophorolipids

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