Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola

Inge Van Bogaert, Guodong Zhang, Jun Yang, Jun Yan Liu, Yonghao Ye, Wim Soetaert, Bruce D. Hammock

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20- hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.

Original languageEnglish (US)
Pages (from-to)3215-3219
Number of pages5
JournalJournal of Lipid Research
Volume54
Issue number11
DOIs
StatePublished - Nov 2013

Fingerprint

Multifunctional Enzymes
Arachidonic Acid
Biosynthetic Pathways
Mixed Function Oxygenases
Bioactivity
Metabolism
Yeast
Cytochrome P-450 Enzyme System
Blood Vessels
Fatty Acids
Yeasts
20-hydroxy-5,8,11,14-eicosatetraenoic acid
Inflammation
Lipids
Oxidation
Molecules

Keywords

  • 20-hydroxyeicosatetraenoic acid
  • Arachidonic acid
  • Sophorolipids

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

Cite this

Van Bogaert, I., Zhang, G., Yang, J., Liu, J. Y., Ye, Y., Soetaert, W., & Hammock, B. D. (2013). Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola. Journal of Lipid Research, 54(11), 3215-3219. https://doi.org/10.1194/jlr.D042226

Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola. / Van Bogaert, Inge; Zhang, Guodong; Yang, Jun; Liu, Jun Yan; Ye, Yonghao; Soetaert, Wim; Hammock, Bruce D.

In: Journal of Lipid Research, Vol. 54, No. 11, 11.2013, p. 3215-3219.

Research output: Contribution to journalArticle

Van Bogaert, I, Zhang, G, Yang, J, Liu, JY, Ye, Y, Soetaert, W & Hammock, BD 2013, 'Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola', Journal of Lipid Research, vol. 54, no. 11, pp. 3215-3219. https://doi.org/10.1194/jlr.D042226
Van Bogaert, Inge ; Zhang, Guodong ; Yang, Jun ; Liu, Jun Yan ; Ye, Yonghao ; Soetaert, Wim ; Hammock, Bruce D. / Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola. In: Journal of Lipid Research. 2013 ; Vol. 54, No. 11. pp. 3215-3219.
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