Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola

Inge Van Bogaert, Guodong Zhang, Jun Yang, Jun Yan Liu, Yonghao Ye, Wim Soetaert, Bruce D. Hammock

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20- hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.

Original languageEnglish (US)
Pages (from-to)3215-3219
Number of pages5
JournalJournal of Lipid Research
Volume54
Issue number11
DOIs
StatePublished - Nov 2013

Keywords

  • 20-hydroxyeicosatetraenoic acid
  • Arachidonic acid
  • Sophorolipids

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

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