Abstract
The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20- hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.
Original language | English (US) |
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Pages (from-to) | 3215-3219 |
Number of pages | 5 |
Journal | Journal of Lipid Research |
Volume | 54 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2013 |
Keywords
- 20-hydroxyeicosatetraenoic acid
- Arachidonic acid
- Sophorolipids
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Endocrinology