Abstract
The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20- hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3215-3219 |
| Number of pages | 5 |
| Journal | Journal of Lipid Research |
| Volume | 54 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 2013 |
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Keywords
- 20-hydroxyeicosatetraenoic acid
- Arachidonic acid
- Sophorolipids
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Endocrinology
Cite this
Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola. / Van Bogaert, Inge; Zhang, Guodong; Yang, Jun; Liu, Jun Yan; Ye, Yonghao; Soetaert, Wim; Hammock, Bruce D.
In: Journal of Lipid Research, Vol. 54, No. 11, 11.2013, p. 3215-3219.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola
AU - Van Bogaert, Inge
AU - Zhang, Guodong
AU - Yang, Jun
AU - Liu, Jun Yan
AU - Ye, Yonghao
AU - Soetaert, Wim
AU - Hammock, Bruce D.
PY - 2013/11
Y1 - 2013/11
N2 - The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20- hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.
AB - The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20- hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.
KW - 20-hydroxyeicosatetraenoic acid
KW - Arachidonic acid
KW - Sophorolipids
UR - http://www.scopus.com/inward/record.url?scp=84885965970&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885965970&partnerID=8YFLogxK
U2 - 10.1194/jlr.D042226
DO - 10.1194/jlr.D042226
M3 - Article
C2 - 23966667
AN - SCOPUS:84885965970
VL - 54
SP - 3215
EP - 3219
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 11
ER -