Preparation, characterization, and surface immobilization of native vesicles obtained by mechanical extrusion of mammalian cells.

Huawen Wu, Ann E. Oliver, Viviane N. Ngassam, Chanel K. Yee, Atul N. Parikh, Yin Yeh

Research output: Contribution to journalArticle

Abstract

Native vesicles or "reduced protocells" derived by mechanical extrusion concentrate selected plasma membrane components, while downsizing complexities of whole cells. We illustrate this technique, characterize the physical-chemical properties of these reduced configurations of whole cells, and demonstrate their surface immobilization and patternability. This simple detergent-free vesicularized membrane preparation should prove useful in fundamental studies of cellular membranes, and may provide a means to engineer therapeutic cells and enable high-throughput devices containing near-native, functional proteolipidic assemblies.

Original languageEnglish (US)
Pages (from-to)685-692
Number of pages8
JournalIntegrative biology : quantitative biosciences from nano to macro
Volume4
Issue number6
StatePublished - Jun 2012
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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