Abstract
Covalently linked cross sections refer to structures that mimic hydrogen- bonded purine-pyrimidine, purine-purine, and pyrimidine-pyrimidine duplexes. Cross sections dA/U and A/dT, which have been synthesized chemically, have molecular dimensions similar to purine-pyrimidine base pairs in a double helix. We propose that antibodies to such covalent cross sections might facilitate the study of the pathogenesis of specific diseases or of biochemical processes in which base pair involvement is suspected and/or demonstrated. We have made polyclonal antibodies against 'A:U' and 'A:T' cross sections by immunizing rabbits with dA/U and A/dT, each conjugated to keyhole limpet hemocyanin (KLH). The antibodies were found to be highly specific for the cross sections and to cross react minimally to single nucleosides. Hybridomas secreting monoclonal antibodies to 'A:T' were then generated from spleen cells of mice immunized with A/dT conjugated to KLH. The MAbs produced were also found to be highly specific for 'A:T' among various nucleosides. In fact, the binding of most of the monoclonal antibodies to 'A:T' was only partially inhibited by high concentrations of adenosine or thymidine. All monoclonal antibodies to 'A:T' cross react, but with lower affinity, to 'A:U.' Selected MAbs showed greater inhibition of binding to 'A:T'-BSA by A + T than by A or T alone.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 499-507 |
| Number of pages | 9 |
| Journal | Hybridoma |
| Volume | 13 |
| Issue number | 6 |
| State | Published - 1994 |
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ASJC Scopus subject areas
- Genetics
- Immunology
Cite this
Preparation and characterization of polyclonal and monoclonal antibodies specific for covalently linked DNA/RNA cross sections. / Liu, Fu-Tong; Shenhav, A.; Bhat, B.; Leonard, N. J.
In: Hybridoma, Vol. 13, No. 6, 1994, p. 499-507.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preparation and characterization of polyclonal and monoclonal antibodies specific for covalently linked DNA/RNA cross sections
AU - Liu, Fu-Tong
AU - Shenhav, A.
AU - Bhat, B.
AU - Leonard, N. J.
PY - 1994
Y1 - 1994
N2 - Covalently linked cross sections refer to structures that mimic hydrogen- bonded purine-pyrimidine, purine-purine, and pyrimidine-pyrimidine duplexes. Cross sections dA/U and A/dT, which have been synthesized chemically, have molecular dimensions similar to purine-pyrimidine base pairs in a double helix. We propose that antibodies to such covalent cross sections might facilitate the study of the pathogenesis of specific diseases or of biochemical processes in which base pair involvement is suspected and/or demonstrated. We have made polyclonal antibodies against 'A:U' and 'A:T' cross sections by immunizing rabbits with dA/U and A/dT, each conjugated to keyhole limpet hemocyanin (KLH). The antibodies were found to be highly specific for the cross sections and to cross react minimally to single nucleosides. Hybridomas secreting monoclonal antibodies to 'A:T' were then generated from spleen cells of mice immunized with A/dT conjugated to KLH. The MAbs produced were also found to be highly specific for 'A:T' among various nucleosides. In fact, the binding of most of the monoclonal antibodies to 'A:T' was only partially inhibited by high concentrations of adenosine or thymidine. All monoclonal antibodies to 'A:T' cross react, but with lower affinity, to 'A:U.' Selected MAbs showed greater inhibition of binding to 'A:T'-BSA by A + T than by A or T alone.
AB - Covalently linked cross sections refer to structures that mimic hydrogen- bonded purine-pyrimidine, purine-purine, and pyrimidine-pyrimidine duplexes. Cross sections dA/U and A/dT, which have been synthesized chemically, have molecular dimensions similar to purine-pyrimidine base pairs in a double helix. We propose that antibodies to such covalent cross sections might facilitate the study of the pathogenesis of specific diseases or of biochemical processes in which base pair involvement is suspected and/or demonstrated. We have made polyclonal antibodies against 'A:U' and 'A:T' cross sections by immunizing rabbits with dA/U and A/dT, each conjugated to keyhole limpet hemocyanin (KLH). The antibodies were found to be highly specific for the cross sections and to cross react minimally to single nucleosides. Hybridomas secreting monoclonal antibodies to 'A:T' were then generated from spleen cells of mice immunized with A/dT conjugated to KLH. The MAbs produced were also found to be highly specific for 'A:T' among various nucleosides. In fact, the binding of most of the monoclonal antibodies to 'A:T' was only partially inhibited by high concentrations of adenosine or thymidine. All monoclonal antibodies to 'A:T' cross react, but with lower affinity, to 'A:U.' Selected MAbs showed greater inhibition of binding to 'A:T'-BSA by A + T than by A or T alone.
UR - http://www.scopus.com/inward/record.url?scp=0028559581&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028559581&partnerID=8YFLogxK
M3 - Article
C2 - 7737674
AN - SCOPUS:0028559581
VL - 13
SP - 499
EP - 507
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
SN - 2167-9436
IS - 6
ER -