Preparation and characterization of a multilayer biomimetic scaffold for bone tissue engineering

Lijun Kong, Qiang Ao, Aijun Wang, Kai Gong, Xi Wang, Guangyuan Lu, Yandao Gong, Nanming Zhao, Xiufang Zhang

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

In scaffold based bone tissue engineering, both the pore size and the mechanical properties of the scaffold are of great importance. However, an increase in pore size is generally accompanied by a decrease in mechanical properties. In order to achieve both suitable mechanical properties and porosity, a multilayer scaffold is designed to mimic the structure of cancellous bone and cortical bone. A porous nano-hydroxyapatite-chitosan composite scaffold with a multilayer structure is fabricated and encased in a smooth compact chitosan membrane layer to prevent fibrous tissue ingrowth. The exterior tube is shown to have a small pore size (15-40 μm in diameter) for the enhancement of mechanical properties, while the core of the multilayer scaffold has a large pore size (predominantly 70-150 μm in diameter) for nutrition supply and bone formation. Compared with the uniform porous scaffold, the multilayer scaffold with the same size shows an enhanced mechanical strength and larger pore size in the center. More cells are shown to grow into the center of the multilayer scaffold in vitro than into the uniform porous scaffold under the same seeding condition. Finally, the scaffolds are implanted into a rabbit fibula defect to evaluate the osteoconductivity of the scaffold and the efficacy of the scaffold as a barrier to fibrous tissue ingrowth. At 12 weeks post operation, affluent blood vessels and bone formation are found in the center of the scaffold and little fibrous tissue is noted in the defect site.

Original languageEnglish (US)
Pages (from-to)223-239
Number of pages17
JournalJournal of Biomaterials Applications
Volume22
Issue number3
DOIs
StatePublished - Nov 2007
Externally publishedYes

Keywords

  • Biomimetic
  • Bone formation
  • Mechanical properties
  • Porosity
  • Tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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