Aim: To fabricate a multi-channeled chitosan nerve conduit of axial arrangement by using novel molds. Methods: The experiment was carried out in the Biomaterials and Tissue Engineering Laboratory of Tsinghua University between May and September in 2004. Porous or imperforate chitosan tubes with 2-5 mm inner diameter and 0.2-1.0 mm wall thickness were made firstly. The stainless steel needles longitudinally perforated through the chitosan tubes that filled with chitosan gel. Lyophilization was used to finish fabrication. Then the physical-chemical properties of the multi-channeled chitosan nerve conduits were characterized, including swelling property and in vitro degradability property. Wright's staining and scanning electromicroscope were applied to detect the growth of N2a cells (Neuroblastoma cells, mouse) in the conduits. Results: 1 After hydration in 0.1 mol/L phosphate buffered solution, the inner diameter of the multi-channeled chitosan nerve conduits had no remarkable change (P > 0.01), and the tube wall thickness increased significantly (P < 0.01). 2 After in vitro degradation in lysozyme solution for 8 weeks, the conduits still remained enough mechanical strength and tenacity, without the change of morphosis. 3 Wright's staining showed that N2a cells, emitting blue, spread out on the surface of the conduits, or clustered within the matrix of the conduits. Scanning electromicroscope revealed the morphology and distribution in conduits of the cells. Conclusion: The multi-channeled chitosan conduits possess suitable swelling property, mechanical strength and nerve cell affinity, indicating potential values for studies and application.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Clinical Rehabilitative Tissue Engineering Research|
|State||Published - Jan 1 2008|
ASJC Scopus subject areas
- Clinical Biochemistry
- Biomedical Engineering